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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety and Processing Research Unit » Research » Publications at this Location » Publication #292278
Title: Effect of bicarbonate concentration on aerobic growth of campylobacter in a fumarate-pyruvate medium

Author
item Hinton Jr, Arthur

Submitted to: International Association for Food Protection Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/9/2013
Publication Date: 7/29/2013
Citation: Hinton Jr, A. 2013. Effect of bicarbonate concentration on aerobic growth of campylobacter in a fumarate-pyruvate medium. International Association for Food Protection. 76(Supplement A) pg. 63.

Interpretive Summary: none

Technical Abstract: The purpose of the present study was to examine the effect of sodium bicarbonate (NaHCO3) concentration on aerobic growth of Campylobacter in a fumarate-pyruvate medium. Fumarate-pyruvate broth medium was supplemented with 0.00 to 0.10% NaHCO3 and inoculated with Campylobacter coli 33559, Campylobacter fetus 27349, Campylobacter jejuni 33560, or Campylobacter jejuni 49349. Aliquots of inoculated media were transferred to wells of a honeycomb plate and placed in a Bioscreen Microbiology Reader. Cultures were incubated aerobically at 37oC for 72 h, and culture optical density (OD) was measured during incubation (n=5). Additional experiments were conducted to compare CFU/ml of Campylobacter recovered from media supplemented with 0.05 % NaHCO3, inoculated with Campylobacter spp., and incubated aerobically or microaerophilically for 72 h at 37oC (n=6). Results indicated that the OD of cultures of all isolates, except C. coli, were significantly (P < 0.05) higher when grown in fumarate-pyruvate medium containing added NaHCO3. The addition of NaHCO3 produced significant increases in the OD of most isolates during early periods of growth. Also, there was a 5 to 6 log increase in CFU/ml of all isolates recovered from media supplemented with 0.05% NaHCO3, and there was no significant difference in the number of CFU/ml recovered from media incubated aerobically or anaerobically.