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Title: Analysis of polyphenols, anthocyanins and carotenoids in tubers from Solanum tuberosum group Phureja, Stenotomum and Andigena

Author
item PILLAI, SYAMKUMAR - Washington State University
item Navarre, Duroy - Roy
item Bamberg, John

Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/10/2013
Publication Date: 9/1/2013
Citation: Pillai, S., Navarre, D.A., Bamberg, J.B. 2013. Analysis of polyphenols, anthocyanins and carotenoids in tubers from Solanum tuberosum group Phureja, Stenotomum and Andigena. American Journal of Potato Research. 90:440-450.

Interpretive Summary: Potatoes have considerable genetic diversity that can be utilized to develop cultivars with even higher amounts of phytonutrients. Based primarily on visual appearance and color, 1500 primitive landraces were narrowed down to 65, which were then analyzed for various phytonutrients. Ten of these genotypes were selected for more in-depth analysis by HPLC. Several genotypes were identified that had exceptionally high amounts of polyphenols, anthocyanins and carotenoids, all compounds known to have health-promoting properties.

Technical Abstract: Sixty-five Solanum tuberosum group Andigena, Phureja and Stenotomum genotypes from an initial population of 1500 were analyzed for phenylpropanoids, carotenoids, and antioxidant capacity. Total phenolic content ranged from 3 to 49 mg/g DW, total carotenoids from 4.1 to 154 µg/g DW, anthocyanins from 0.27 to 34 mg/g DW and antioxidant capacity from 60-1,767 µmol TE/g DW. HPLC analysis of phenolic extracts revealed that the chlorogenic acid being the most abundant polyphenol in all the genotypes studied. Ten genotypes were independently grown out for more in-depth phytonutrient analysis. The Phureja genotypes RN 27.01 had the highest polyphenol, anthocyanin and antioxidant content, while RN 39.05 had the highest carotenoid content. The tuber percentage dry matter varied markedly among the ten genotypes, influencing the phytonutrient values when expressed on a dry weight basis. Chlorogenic acid ranged from 1.7-29.4 mg/g DW and kaempferol-3-rutinose up to 3 mg/g DW. Petunidin-3-O-coum-rutinoside-5-O-glu or pelargonidin-3-O-coum-rutinoside-5-O-glu were the most abundant anthocyanins. The principal carotenoids were lutein, zeaxanthin, violaxanthin, and antheraxanthin, but no specific carotenoid was predominant in all genotypes. These findings further support utilization of Phureja group germplasm for phytonutrient enhancement efforts.