Purification of glucosinolates from Camelina sativa seeds

Authors: Berhow, Mark; POLAT, UMIT - Uludag University; GLINSKI, JAN - Planta Analytica, Llc; GLENSK, MICHAL - Planta Analytica, Llc; Vaughn, Steven; Isbell, Terry; AYALA-DIAZ, IVAN - Iowa State University; MAREK, LAURA - Iowa State University; Gardner, Candice

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2011
Publication Date: 12/15/2011
Citation: Berhow, M.A., Polat, U., Glinski, J., Glensk, M., Vaughn, S.F., Isbell, T., Ayala-Diaz, I., Marek, L., Gardner, C.A. 2011. Purification of glucosinolates from Camelina sativa seeds. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Camelina sativa L. Crantz defatted seed press cake contains a number of phytochemicals, including the flavonoid rutin (quercetin 3-O-rutinoside), an acylated quercetin glycoside, and three glucosinolates: glucoarabin (9-(methylsulfinyl)nonyl-glucosinolate) glucocamelinin (10-(methylsulfinyl)decyl-glucosinolate), and 11-(methylsulfinyl)undecyl-glucosinolate. We have developed methods to isolate mg quantities of these glucosinolates and flavonoids using a combination of reverse phase flash countercurrent chromatography and preparative HPLC methods. An analytical method has been developed to accurately measure glucosinolate levels in seeds, seed meal and sprouts. The camelina glucosinolates have not been assessed for their phytochemical activities in agriculture or their nutritional/pharmacological roles in animals due to the lack of availability of purified standards for study. Sprouted camelina seeds were shown to have decreasing levels of glucosinolates over seven days of sprouting. This study will be used to develop new uses for camelina seeds and seed meals based on glucosinolate content.