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ARS Home » Southeast Area » Florence, South Carolina » Coastal Plain Soil, Water and Plant Conservation Research » Research » Publications at this Location » Publication #262751

Title: Simultaneous quantification of oil and protein in cottonseed by low-field time-domain nuclear magnetic resonance

Author
item HORN, PATRICK - University Of North Texas
item NEOGI, PURNIMA - University Of North Texas
item TOMBOKAN, XENIA - Bruker Optics
item GHOSH, SUPRIYO - Bruker Optics
item Campbell, Benjamin - Todd
item CHAPMAN, KENT - University Of North Texas

Submitted to: Journal of the American Oil Chemists' Society
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/5/2011
Publication Date: 4/30/2011
Citation: Horn, P.J., Neogi, P., Tombokan, X., Ghosh, S., Campbell, B.T., Chapman, K.D. 2011. Simultaneous quantification of oil and protein in cottonseed by low-field time-domain nuclear magnetic resonance. Journal of the American Oil Chemists' Society. 88:1521-1529.

Interpretive Summary: Modification of cottonseed quality traits is likely to be achieved through a combination of genetic modification, manipulation of nutrient allocation and selective breeding. Oil and protein stores comprise the majority of mass of cottonseed embryos. A more comprehensive understanding of the relationship between lint quality, lint yield and embryo reserve accumulation will help assist breeders in their efforts to improve seed value. Here we report the development of a rapid, non-destructive, simultaneous method for quantifying oil and protein levels within cottonseed. This approach is suitable for a minimal amount of seed and represents an accurate, non-invasive alternative to conventional, time consuming chemical extractions. We demonstrate the value of this approach by surveying seed reserve content, identifying extremes of either protein and/or oil, in two sets of diverse germplasm.

Technical Abstract: Modification of cottonseed quality traits is likely to be achieved through a combination of genetic modification, manipulation of nutrient allocation and selective breeding. Oil and protein stores comprise the majority of mass of cottonseed embryos. A more comprehensive understanding of the relationship between lint quality, lint yield and embryo reserve accumulation will help assist breeders in their efforts to improve seed value. Here we report the development of a rapid, non-destructive, simultaneous method for quantifying oil and protein levels within cottonseed by low-field 1H time-domain nuclear magnetic resonance (TD-NMR). This approach is suitable for a minimal amount of seed and represents an accurate (oil R2 = 0.998, protein R2 = 0.95), non-invasive alternative to conventional, time consuming chemical extractions. We demonstrate the value of this approach by surveying seed reserve content, identifying extremes of either protein and/or oil, in two sets of diverse germplasm.