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Title: Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle than continuous feeding in neonatal pigs

Author
item GAZZANEO, MARIA - Children'S Nutrition Research Center (CNRC)
item MURGAS-TORRAZZA, ROBERTO - Children'S Nutrition Research Center (CNRC)
item SURYAWAN, AGUS - Children'S Nutrition Research Center (CNRC)
item ORELLANA, RENAN - Children'S Nutrition Research Center (CNRC)
item SRIVASTAVA, NEERAJ - Children'S Nutrition Research Center (CNRC)
item NGUYEN, HANH - Children'S Nutrition Research Center (CNRC)
item WILSON, FIONA - Children'S Nutrition Research Center (CNRC)
item FIOROTTO, MARTA - Children'S Nutrition Research Center (CNRC)
item DAVIS, TERESA - Children'S Nutrition Research Center (CNRC)

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 2/14/2010
Publication Date: 4/24/2010
Citation: Gazzaneo, M.C., Murgas-Torrazza, R., Suryawan, A., Orellana, R.A., Srivastava, N., Nguyen, H.V., Wilson, F.A., Fiorotto, M.L., Davis, T.A. 2010. Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle than continuous feeding in neonatal pigs [abstract]. Federation of American Societies for Experimental Biology Conference, Session: Animal research models in macronutrient metabolism I, April 24-28, 2010, Anaheim, California. 24: 327.3.

Interpretive Summary:

Technical Abstract: Feeding by orogastric tube, using either continuous or intermittent bolus delivery, is common for infants for whom normal feeding is contraindicated. To determine the impact of different feeding modalities on muscle protein anabolism, neonatal pigs (5–7 day old) received a balanced enteral formula either by intermittent bolus (B, n=7) every four hours, or were continuously fed (C, n=5). Fractional protein synthesis rates and translational control mechanisms in skeletal muscle were examined at 0, 24, and 25.5 hours. Plasma amino acid and insulin levels were modestly increased in the continuously fed group and in the bolus group mimicked the pulsatile pattern of meal feeding. Feeding increased protein synthesis by 53% in the continuous group, by 63% in the bolus group before a meal, and by 126% after a bolus feed. The activation of eukaryotic initiation factor (eIF) 4E binding protein (4EBP1), rp S6 kinase 1 (S6K1), protein kinase B (PKB), dissociation of 4EBP1-eIF4E complex, and formation of the active eIF4E-eIF4G complex were unaffected by continuous feeding, but were increased more than 10 fold after a bolus meal. These results suggest that intermittent bolus feeding increases muscle protein synthesis to a greater extent than continuous feeding by stimulating translation initiation.