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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #253239
Title: Starch granule formation and protein deposition in wheat (Triticum aestivum L.) starchy endosperm cells is altered by high temperature during grain fill

Author
item Hurkman Ii, William
item Wood, Delilah - De

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 5/1/2010
Publication Date: 6/2/2010
Citation: Hurkman II, W.J., Wood, D.F. 2010. Starch granule formation and protein deposition in wheat (Triticum aestivum L.) starchy endosperm cells is altered by high temperature during grain fill. Scanning 2010, Monterey, CA, May 17-19, 2010. 7729:58.

Interpretive Summary:

Technical Abstract: High temperatures during wheat grain fill decrease starch and protein levels, adversely affecting wheat yield and flour quality. To determine the effect of high temperature on starchy endosperm cell development, grain (Triticum aestivum L. "Butte 86") was produced under a 24/17°C or 37/28°C day/night regimen imposed from flowering to maturity and starch and protein deposition examined using fluorescence and scanning electron microscopy. The high temperature regimen shortened the duration of grain fill from 40 to 18 days. Under the 37/28°C regimen, A-type starch granules increased in number and decreased in size and B-type granules decreased in both number and size. A-type starch granules in mature grain exhibited pitting, suggesting enhanced action of starch degradative enzymes. Under both temperature regimens, protein bodies originated early in development and coalesced during mid to late development to form a continuous protein matrix surrounding the starch granules. Under the 37/28oC regimen, the proportion of protein matrix increased in endosperm cells of mature grain. Taken together, the changes in starch granule number and size and in protein matrix amount provide clues for understanding how high temperature during grain fill can affect end use properties of wheat flour.