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Submitted to: Developmental and Comparative Immunology
Publication Type: Proceedings Publication Acceptance Date: 3/16/2009 Publication Date: 8/30/2009 Citation: Waldbieser, G.C. 2009. Expression Profiling Using New Generation Sequencing Technologies. Developmental and Comparative Immunology. P: 305 Interpretive Summary: Microarray hybridization technology has become widely used in parallel analysis of gene expression. Recent advances in genome sequencing platforms point to an alternate approach through digital quantitation of sequencing reads produced from cDNA samples. This presentation will compare advantages and limitations of transcript profiling using existing and emerging high-throughput sequencing platforms versus microarray hybridization approaches. Technical Abstract: Microarray hybridization technology has become widely used in parallel analysis of gene expression. Recent advances in genome sequencing platforms point to an alternate approach through digital quantitation of sequencing reads produced from cDNA samples. This presentation will compare advantages and limitations of transcript profiling using existing and emerging high-throughput sequencing platforms versus microarray hybridization approaches. |