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Title: Sterol Regulatory Transcription Factor-1: Key Regulator of Fasting Response in the Adipose Tissue inGPigs

Author
item LKHAGVADORJ, SENDER - IOWA STATE UNIV
item QU, LONG - IOWA STATE UNIV
item CAI, WEIGUO - IOWA STATE UNIV
item COUTURE, OLIVER - IOWA STATE UNIV
item WANG, YAN FANG - IOWA STATE UNIV
item Barb, Claude
item Hausman, Gary
item REKAYA, ROMDHUNE - UGA
item NETTLETON, DANIEL - IOWA STATE UNIV
item ANDERSON, LLOYD - IOWA STATE UNIV
item DEKKERS, JACK - IOWA STATE UNIV
item TUGGLE, CHRISTOPHER - IOWA STATE UNIV

Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 1/9/2008
Publication Date: 4/10/2008
Citation: Lkhagvadorj, S., Qu, L., Cai, W., Couture, O.P., Wang, Y., Barb, C.R., Hausman, G.J., Rekaya, R., Nettleton, D.S., Anderson, L.L., Dekkers, J.C., Tuggle, C.K. 2008. Sterol Regulatory Transcription Factor-1: Key Regulator of Fasting Response in the Adipose Tissue inGPigs [abstract]. Experimental Biology. 22:1205.6

Interpretive Summary: none.

Technical Abstract: The genetic mechanisms controlling appetite and feeding behaviors are not well understood. In this study, transcriptional profiling was used to identify porcine genes and pathways that respond to a fasting treatment or to a missense mutation (D298N) in the melanocortin-4 receptor (MC4R) gene, which has been associated with increased growth and feed efficiency. Prepubertal gilts (n=24) homozygous for D298N MC4R or wildtype were either fed ad lib. or fasted for 3 days in a completely randomized block design with 2x2 factorial treatment structure. The Affymetrix Porcine Genome was used to profile gene expression of liver and subcutaneous fat. Due to fasting, 7,029 genes in adipose tissue and 1,831 genes in liver were declared differentially expressed (q<0.05), but an effect of MC4R was not observed on expression in both tissues under same criterion. Pathway analyses indicated that 23 of the tested set of 298 genes that were downregulated (p<0.05, fold change>2) in the adipose tissue due to fasting were directly regulated by sterol regulatory element binding transcription factor-1 (SREBF1). We confirmed the expression levels of SREBF1 and of its targets such as fatty acid synthase, aconitase-1, acetyl CoA carboxylase alpha, and acetyl CoA synthase in the adipose tissue by qPCR. Results indicate that SREBF1 may play a key role in determining the pathways that respond to fasting in pigs.