Author
 |
Roehrdanz, Richard |
|
Submitted to: Genbank
Publication Type: Other Publication Acceptance Date: 10/22/2007 Publication Date: 10/27/2007 Citation: Roehrdanz, R.L. 2007. Sequences of Wolbachia wsp genes reveal multiple infection of individual northern corn rootworms (Diabrotica barberi) by several Wolbachia strains. Genbank. Accession numbers: EU188677-EU188725. Interpretive Summary: Technical Abstract: Northern corn rootworm (Diabrotica barberi)(NCR) populations in the USA are infected with at least 4 strains of the endosymbiont, Wolbachia. NCR from eastern Illinois to Pennsylvania appear to harbor at least 4 different strains designated wBar1, wBar3, wBar4, and wBar5. NCR from central Illinois appears to be singly infected with a strain wBar2. NCR from western Illinois through the Great Plains are uninfected. Multiple infection was detected by sequencing multiple clones of the Wolbachia wsp gene from the same insect. Five clades are indicated by sequence comparison, length, and Tsp509I restriction patterns. The segments range from 582 to 603 bp. The wsp gene segment is composed of 4 hypervariable regions (HV) interspersed by more conserved regions. Different Wolbachia strains may have alternative mixes of these HV regions. wBar1 and wBar2 have completely different HVs. wBar3 is defined by a 3rd complete set of HVs while wBar4 appears to be unique for HV2-HV4. The latter was found with both wBAr1 and wBar2 HV1. A single alternate HV2 signals a possible 5th Wolbachia strain. The presence of pieces of different HV regions in a single insect indicates multiple Wolbachia infections. An NCR from OH has HV regions from all 4 primary strains. It remains to be determined if a 4X (or greater) multiple infection is the rule or an exception. EU188677, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi AR2121W1 EU188678, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi OH1656W3 EU188679, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2398W6 EU188680, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2399W3 EU18868 , Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2399W4 EU188682, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2398W1 EU188683, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi AR2121W6 EU188684, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2400W1 EU188685, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi OH1656W1 EU188686, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi RS1698W5 EU188687, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2399W1 EU188688, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2399W6 EU188689, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi OH1656W4 EU188690, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi OH1656W5 EU188691, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2398W5 EU188692, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi RS1698W1 EU188693, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi OH1656W6 EU188694, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi WI2409W3 EU188695, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2400W5 EU188696, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2400W6 EU188697, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi AR2121W5 EU188698, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi OH1656W2 EU188699, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi PR2399W2 EU188701, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi WI2409W4 EU188702, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi WI2409W5 EU188703, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi CA2763W1 EU188704, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi CA2763W2 EU188705, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi CA2763W3 EU188706, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi CA2763W5 EU188707, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi CA2763W6 EU188708, Wolbachia, wsp gene, endosymbiont of Diabrotica barberi CA2763W7 EU188709, Wolbac |
