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Title: The IRE1/bZIP60 pathway are activated by potexvirus and potyvirus small membrane binding proteins

Author
item VERCHOT, JEANMARIE - Oklahoma State University
item Halterman, Dennis
item ARIAS, OMAR - Oklahoma State University
item PENA, ELISABETH - Oklahoma State University
item VELA ARIAS, ALEXIS - Oklahoma State University

Submitted to: Molecular Plant-Microbe Interactions
Publication Type: Abstract Only
Publication Acceptance Date: 2/29/2016
Publication Date: 7/17/2016
Citation: Verchot, J., Halterman, D.A., Arias, O., Pena, E., Vela Arias, A. 2016. The IRE1/bZIP60 pathway are activated by potexvirus and potyvirus small membrane binding proteins [abstract]. Molecular Plant-Microbe Interactions. Paper No. P7-225.

Interpretive Summary:

Technical Abstract: The endoplasmic reticulum provides an environment for protein synthesis, folding and distribution to all corners of the cell. With respect to protein synthesis and folding, quality production is central to maintaining homeostasis. When conditions occur that disrupt the folding capacity of the ER causing misfolded or unfolded proteins to accumulate, the ER becomes stressed. Transmembrane proteins embedded in the ER with luminal domains can act as ER stress sensors. The unfolded protein response (UPR) is a signaling cascade that is activated by ER stress and several downstream factors function to help the cell adapt to circumstances and restore ER homeostasis. Positive strand RNA viruses often depend upon the ER for replication, translation, protein processing and intercellular transport. Viruses can cause transient, chronic or acute ER stress and interaction with the unfolded protein response (UPR) machinery dictates whether cells become tolerant to infection or achieve a state of unresolvable stress that is marked by cell death. The potexvirus TGB3 movement protein and the potyvirus 6K2 protein both reside in the ER. These proteins stimulate the ER stress sensor IRE1 which splices the bZIP60 mRNA. The truncated bZIP60 transcription factor stimulates expression of ER stress related genes. We determine that the IRE1/bZIP60 pathway contributes to disease regulation and virus systemic infection in plants. Complete understanding of these mechanisms will lead to novel strategies to improve plant tolerance to virus infection.