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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #323251
Title: Progress toward the development of diploid recombinant inbred lines

Author
item Jansky, Shelley
item DOUCHES, DAVID - Michigan State University
item ENDELMAN, JEFF - University Of Wisconsin

Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 11/17/2015
Publication Date: 1/8/2016
Citation: Jansky, S.H., Douches, D., Endelman, J. 2016. Progress toward the development of diploid recombinant inbred lines [abstract]. Plant and Animal Genome. Paper No. P1075.

Interpretive Summary:

Technical Abstract: Recombinant inbred lines (RILs) offer new opportunities for mapping traits in potato. They also provide an opportunity to evaluate responses to inbreeding in interspecific hybrids. We are developing a set of six RILs, which will comprise a nested association mapping population. The common parent is M6, an inbred line of the diploid wild relative Solanum chacoense. It is homozygous for a dominant self-incompatibility inhibitor. Other germplasm in the RILs includes S. tuberosum Tuberosum Group, S. tuberosum Phureja Group, and S. berthaultii. The parents of the RILs have been selected based on tuber morphology and yield, disease resistance, and processing quality. We plan to create 100 F6 inbred lines of each of the six RIL populations. Currently, we have populations ranging from the F2 to F6 generations. A large number of F5 plants derived from DM1-3 x M6 produce very long tubers, even though no selection for tuber traits has been carried out. During inbreeding, a reduction in vigor has been observed, as expected. However, the major challenge is maintaining adequate flower production and fertility for seed production. The RILs will be shared with the research community for phenotyping and mapping.