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Title: Diversity of parasitic fungi from soybean cyst nematode associated with long-term continuous cropping of soybean

Author
item SONG, JIE - Northeast Institute Of Geography And Agronomy, Cas
item Li, Shuxian
item XU, YANLI - Chinese Academy Of Sciences
item WEI, WEI - Chinese Academy Of Sciences
item YAO, QIN - Chinese Academy Of Sciences
item PAN, FENGJUAN - Chinese Academy Of Sciences

Submitted to: Acta Agriculture Scandinavica Section B – Soil & Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/15/2016
Publication Date: 5/23/2016
Citation: Song, J., Li, S., Xu, Y., Wei, W., Yao, Q., Pan, F. 2016. Diversity of parasitic fungi from soybean cyst nematode associated with long-term continuous cropping of soybean. Acta Agriculture Scandinavica Section B – Soil & Plant Science. 66:432-442.

Interpretive Summary: The soybean cyst nematode (SCN) is a major yield-limiting pest of soybean. In this study, experiments were conducted to examine the parasitic fungi from SCN associated with disease-suppressive and non-suppressive soil fields in Northeast China. Several well- known bio-control fungi were isolated from SCN. The numbers of those fungi isolated from disease-suppressive field were significantly higher than that in the non-suppressive soil fields. Long-term cropping with soybean monoculture in the black soil field might provide soil environments favorable for parasitic fungi growing on SCN. Those parasitic fungi may play an important role in suppressing SCN in the suppressive soil.

Technical Abstract: The soybean cyst nematode (SCN) is a major yield-limiting pest of soybean. In this study, experiments were conducted to examine the parasitic fungi from SCN associated with disease-suppressive and non-suppressive soil fields in Northeast China. Soil samples were collected from three fields under different rotation systems: (i) a continuous long-term cropping field with soybean from 1991 to 2014 (SSSS). It was a disease-suppressive field identified previously, (ii) cycles of three-year rotation with corn, soybean, and wheat from 1991 to 2014 (WCS), and (iii) a continuous cropping field with soybean for 2 y from 2012 to 2014 (SSC). The known bio-control fungi, such as Pochonia chlamydosporia and Paecilomyces lilacinus were isolated from SCN. The numbers of those fungi isolated from the SSSS field were significantly (P < 0.05) higher than that in the other two fields. Principal component analysis of the PCR-denaturing gradient gel electrophoresis (PCR-DGGE) data revealed that fungal communities on cysts could be divided into three groups: one group occurred in SSSS, and the other two groups were in WCS and SSC fields, respectively. Long-term cropping with soybean monoculture in the black soil field might provide soil environments favorable for parasitic fungi growing on SCN. Those parasitic fungi may play an important role in suppressing SCN in the suppressive soil.