Iron status of channel catfish Ictalurus punctatus affected by channel catfish anemia and response to parenteral iron

Authors: CAMUS, ALVIN - University Of Georgia; WISE, DAVID - Mississippi State University; KHOO, LESTER - Mississippi State University; JISHU, SHI - Kansas State University; BERGHAUS, ROY - University Of Georgia

Submitted to: Diseases of Aquatic Organisms
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/30/2013
Publication Date: 1/6/2014
Citation: Camus, A.C., Wise, D.J., Khoo, L.H., Jishu, S., Berghaus, R.D. 2014. Iron status of channel catfish Ictalurus punctatus affected by channel catfish anemia and response to parenteral iron. Diseases of Aquatic Organisms. 107:241-248.

Interpretive Summary: This study confirmed that catfish with channel catfish anemia are hypoferrimic and have low packed cell volume, serum and liver iorn as well as low percent transferrin saturation which is consistent with an iron deficiency anemia. Parenteral iron administration produces complete recovery. The cause(s) of the hypoferremia remains unknown.

Technical Abstract: Originally reported in 1983, channel catfish anemia (CCA), also ‘white lip’ or ‘no blood,’ is a major idiopathic disease affecting commercial production in the Mississippi Delta region of the USA. Affected individuals are characterized by lethargy, anorexia, extreme pallor, and packed cell volumes often below 5%, but a definitive cause for CCA remains elusive. Records from the National Warm water Aquaculture Center (NWAC) reveal that, on average, CCA accounted for 4.7% of case submissions from 1994 to 2012. Known infectious agents, parasites, and perturbations in commonly measured water quality variables have been largely excluded, and research has focused on potential feed-related etiologies, particularly folic acid deficiency. No natural or anthropogenic contaminants have been found in feeds, and no associations have been made to any particular feed brand or formulation, or to the age or condition of the feed itself. Contrary to reports indicating a short clinical course, NWAC records indicate an insidious condition where certain ponds have contained fish diagnosed with CCA for up to 4 consecutive years and individual outbreaks have persisted for at least 5 mo. Investigation into the iron status of CCA affected fish revealed values consistent with iron deficiency anemia, including low-packed cell volume (mean ± SE, 5.6 ± 1.0 vs. 24.8 ± 2.4%), serum iron (35.2 ± 3.5 vs. 104.4 ± 18.5 µg dl-1), liver iron (12.2 ± 2.6 vs. 23.3 ± 4.6 µg g-1), and percent transferrin saturation (14.5 ± 2.7 vs. 26.9 ± 3.1%) in anemic and healthy controls, respectively. Administration of parenteral iron produced complete recovery and returned iron indices to within the ranges of normal controls. Despite these findings, factors predisposing a state of hypoferremia remain unknown.