Skip to main content
ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Publications at this Location » Publication #318245
Title: Mapping four quantitative trait loci associated with type I Fusarium head blight resistance in winter wheat ‘INW0412’

Author
item SUN, J - Purdue University
item OHM, H - Purdue University
item POLAND, J - Kansas State University
item Williams, Christie

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/10/2015
Publication Date: 4/29/2016
Citation: Sun, J., Ohm, H.W., Poland, J.A., Williams, C.E. 2016. Mapping four quantitative trait loci associated with type I Fusarium head blight resistance in winter wheat ‘INW0412’. Crop Science. 56:1163-1172. doi:10.2135/cropsci2015.06.0390.

Interpretive Summary: Fusarium head blight is a devastating disease of wheat worldwide. Resistance that limits initial infection is greatly needed but not commonly studied. We identified and mapped the chromosomal positions of 4 new quantitative trait loci that are effective in decreasing initial infection of wheat. Markers for use with these loci during marker-assisted breeding have been developed. The markers and their associated resistance loci can be combined and pyramided with other resistance loci for improving wheat resistance in commercial and private breeding programs.

Technical Abstract: Fusarium head blight (FHB) has become one of the most damaging wheat diseases in humid and semi-humid regions around the world. Breeding efforts have focused on resistance mechanisms that limit the spread once a spike is infected, or type II resistance. But resistance to initial infection, type I resistance, is an important trait that could be combined with other modes of resistance. The objective of this project was to identify and map the locations of previously unknown quantitative trait loci that reduce the incidence of initial infection. A mapping population of 198 recombinant inbred lines (RILs) was evaluated for type I resistance in both the field and greenhouse. A two-enzyme genotyping-by-sequencing (GBS) approach was applied to construct a 1,883 cM linkage map yielding single nucleotide polymorphism (SNP) markers. Composite interval mapping analysis detected a quantitative trait locus (QTL) on chromosome 1AS under greenhouse conditions, and three other QTL on chromosomes 1BL, 2BL, and 3AS in field environments. Each QTL explained between 7.44% and 12.20% of the total phenotypic variation. RILs with markers for all three QTL on chromosomes 1BL, 2BL, and 3AS exhibited significantly improved type I resistance (33.06% improvement) over RILs with none of the three markers in field experiments. Our results also confirmed that type I and type II FHB resistance were controlled by different loci in the wheat RIL population and could be used in combination to provide multiple layers of defense for cultivar development through marker-assisted selection.