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Title: Differences in leukocyte differentiation molecule abundances on domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) neutrophils identified by flow cytometry

Author
item Highland, Margaret
item Schneider, David
item White, Stephen
item MADSEN-BOUTERSE, SALLY - Washington State University
item Knowles Jr, Donald
item DAVIS, WILLIAM - Washington State University

Submitted to: Comparative Immunology Microbiology and Infectious Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/12/2016
Publication Date: 4/14/2016
Citation: Highland, M.A., Schneider, D.A., White, S.N., Madsen-Bouterse, S.A., Knowles Jr, D.P., Davis, W.C. 2016. Differences in leukocyte differentiation molecule abundances on domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) neutrophils identified by flow cytometry . Comparative Immunology Microbiology and Infectious Diseases. DOI: 10.1016/j.cimid.2016.04.006.

Interpretive Summary: Bighorn and domestic sheep pneumonia is production and population limiting. Previous work has indicated bighorn sheep are more susceptible to certain pathogens and therefore pneumonia compared to domestic sheep. An important part of the innate immune system is the neutrophil and the neutrophil is important in pneumonia of bighorn and domestic sheep. This work tested for a difference in certain neutrophil markers that predict responses to pathogens. A difference in markers was found between the two species. Additional work will determine the importance of these markers in response to specific pathogens.

Technical Abstract: Abundance was assessed by utilizing a panel of cross-reactive monoclonal antibodies (mAbs) tested in this study. Characterization of multichannel autofluorescence of eosinophils permitted cell-type specific gating of granulocytes for quantification of LDMs on neutrophils and eosinophils by indirect, single-label, immunofluorescence flow cytometry. Interspecies analyses of isolated leukocytes revealed higher abundance of several '2 integrin subunits (CD11a, CD11b, and CD18) and signal regulatory protein ' (CD172a) on DS neutrophils and of the lipopolysaccharide receptor CD14 on BHS neutrophils (all P<0.5). Concavalin A stimulated lymphocytes from BHS had a higher abundance of the IL-2 receptor, CD25, as compared to DS (P<0.5). This study describes 47 cross-reactive mAbs and provides novel information for elucidating the susceptibility difference to bacterial pneumonia in DS and BHS.