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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #315989
Title: Genetic diversity and serological specificity of emerging cucumber green mottle mosaic virus and development of a broad spectrum LAMP assay

Author
item Ling, Kai-Shu
item Li, Rugang

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/12/2015
Publication Date: 8/30/2015
Citation: Ling, K., Li, R. 2015. Genetic diversity and serological specificity of emerging cucumber green mottle mosaic virus and development of a broad spectrum LAMP assay. 5th Conference of the International Working Group on Legune and Vegetable Viruses. Meeting Abstract. P. 50.

Interpretive Summary: N/A

Technical Abstract: Cucumber green mottle mosaic virus (CGMMV), first described in England, 1935, is a well-known, seed-borne Tobamovirus on cucurbits in Asia, Europe, and the Middle East. The recent outbreaks of CGMMV in Australia and North America (Canada and U.S.) received great concerns from the vegetable seed companies and cucurbit industries. With the increasing activities in off-shore hybrid seed production and global seed trade, sensitive and reliable seed health assay to CGMMV is imperative to ensure that cucurbit growers are using certified virus-free seed lots. However, the presence of genetic diversity with two distinct genotypes (Asian and European) and recent understanding of serological specificity among CGMMV isolates earned concerns for accurate disease diagnosis using serological tests (ELISA or Lateral flow device). Genetic analysis using the complete genome sequence from Canada revealed a strong sequence identity (99%) with numerous Asian isolates, but with only 90% to several European isolates. Genetic diversity was also observed in other CGMMV isolates, i.e., 99% identity between Australia and Asian isolates and 95% identity between California and European isolates. The poor serological reactivity between these two major genotypes made some commercial ELISA kits not reliable for seed health assay. To improve sensitivity and reliability for broad spectrum detection of CGMMV isolates in all genotypes, a loop-mediated isothermal amplification (LAMP) was developed and used to monitor CGMMV infection on greenhouse cucumber. This simple assay would provide a timely and reliable detection of CGMMV in plant health and seed health tests.