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Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only Publication Acceptance Date: 3/15/2015 Publication Date: 7/25/2015 Publication URL: http://www.apsnet.org/meetings/Documents/2015_meeting_abstracts/aps2015abO242.htm Citation: Tatineni, S. 2015. Role of wheat streak mosaic virus-encoded proteins in disease development. American Phytopathological Society Annual Meeting. 90-O. Interpretive Summary: Technical Abstract: Eriophyid mite-transmitted wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are the type species of Tritimovirus and Poacevirus genera, respectively, in the family Potyviridae. TriMV and WSMV exhibit differential symptom phenotypes on wheat: TriMV elicits mild mosaic and mottling while WSMV induces severe chlorotic streaks, leaf yellowing and stunting of plants. Co-infection of wheat by WSMV and TriMV causes synergistic interaction with an increased symptom phenotype. The role of WSMV- and TriMV-encoded proteins in disease development is unknown. In this study, the role of WSMV proteins in disease development was examined by engineering individual WSMV cistrons in pTriMV between P1 and HC-Pro cistrons. Wheat plants infected with TriMV vectors expressing WSMV cistrons were examined for symptom phenotype. TriMV expressing WSMV 6K1, NIa-VPg, NIa-Pro or CP induced more severe symptoms than wild-type TriMV. Expression of WSMV 6K1 in TriMV induced severe mosaic and leaf yellowing, NIa-VPg and CP induced severe chlorotic streaks, mosaic and leaf deformation with moderate stunting of plants, and NIa-Pro induced severe stunting with shortened internodes with dark-green leaves. TriMV with WSMV P1, HC-Pro, P3, CI, 6K2, or NIb induced symptoms similar to those of the wild-type TriMV. These data suggest that WSMV 6K1, NIa-VPg, NIa-Pro, and CP enhanced the symptom phenotype of TriMV, and these proteins might be involved in disease development of WSMV. |
