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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 6/25/2014 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: METHODS: PolyZone (PZ)(Deltamethrin 4.75% AI) was mixed with water according to label instructions (45.4 ml per 3.79 liters H2O = 0.06% solution) for application to 10 x 10 cm squares of blue Trigger-Royal Box fabric (65% polyester and 35% cotton, Galey & Lord, Inc., New York). PZ was applied to run off on 4 cloth squares which were then weathered outdoors in the sun and on 4 additional cloth squares which were then stored in plastic bags in the laboratory. PZ was applied with calibrated plastic trigger-grip sprayers while cloths were suspended on a small clothes line. Care was taken to shake the formulated PZ prior to use. An additional 8 cloth squares, also on a separate clothes line, were sprayed with similar unused sprayers to run off with water to serve as untreated controls. All cloths were air dried prior to use. All tests were begun 24 hours after treatments were applied. After each test, 8 cloth squares (4 treated and 4 untreated) were returned to the clothesline outside. Colony-reared 3- to 5-day-old stable flies (mixed sex) and house flies (females only) were used for the tests. To conduct a test, cloth squares were placed individually on sheets of white printer paper on the laboratory bench. Each cloth square was covered with a 15-cm or larger clear plastic saucer (used for potted plants). One group at a time, flies were knocked down with CO2, counted into groups of 15, and placed onto the respective cloths under the saucers. After either 15 or 30 seconds, the cloth was quickly but gently pulled out from under the saucer and out of contact with the flies. Mortality was recorded 1, 15, 30, 45, 60 minutes, 4 and 24 hours after cloths were removed from fly contact. Tests were conducted weekly until mortality was < 100% at 1 hour post exposure. Flies still alive after 4 hours were supplied with a cotton ball moistened with a 10% sucrose solution. For stable flies, three separate sets of cloths were tested from Aug 7-Sept 11, 2013, Sept 18-Nov 21, 2013, and Dec 12, 2013-Feb 22, 2014. For house flies, one set of cloths was tested from Mar 5-6, 2014. RESULTS: In the first test, there was obviously some contamination, as indicated by the mortality in the controls. This was eliminated in subsequent weekly tests. During the evaluation of the second set of cloths, the government was shut down for 3 weeks. This is reflected in the data tables. Cloths were still weathered during this period. Residuals in cloths stored inside were slightly better than those in cloths stored outside; however, stable flies in all tests were dead by 24 hours post exposure. House flies were not affected by the treatments. Residuals would probably kill stable flies for a few more weeks with increased mortality times. Acknowledgement: Many thanks to Dr. Doug Ross, Bayer Animal Health, for providing the PolyZone for these evaluations. |
