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United States Department of Agriculture

Agricultural Research Service

Research Project: Multidisciplinary Approaches to Enhanced Sugar Beet Germplasm

Location: Sugarbeet Research

Title: Identification and validation of a SNP marker linked to the gene HsBvm-1 for nematode resistance in sugar beet

Authors
item Stevanato, Piergiorgio -
item Trebbi, Daniele -
item Panella, Leonard
item Richardson, Kelley
item Pakish, Linda
item Fenwick, Ann
item Broccanello, Chiara -
item Saccomani, Massimo -

Submitted to: Plant Molecular Biology Reporter
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 28, 2014
Publication Date: July 25, 2014
Repository URL: http://link.springer.com/article/10.1007%2Fs11105-014-0763-8#page-1
Citation: Stevanato, P., Trebbi, D., Panella, L.W., Richardson, K.L., Pakish, L.M., Fenwick, A.L., Broccanello, C., Saccomani, M. 2014. Identification and validation of a SNP marker linked to the gene HsBvm-1 for nematode resistance in sugar beet. Plant Molecular Biology Reporter. DOI: 10.1007/s11105-014-0763-8.

Interpretive Summary: The beet-cyst nematode is one of the major pests of sugar beet. The identification of molecular markers associated with nematode resistance would be helpful for developing resistant varieties. The aim of this study was the identification of molecular markers for the nematode resistance from the resistant sea beet (wild beet), WB242. A population that had both resistant and susceptible individuals was used to find markers. The marker, named SNP_192, showed a complete association with nematode resistance. This association was further validated on a set of resistant and susceptible varieties. We conclude that SNP_192 is a valuable marker for high-throughput marker-assisted breeding of nematode resistance in sugar beet.

Technical Abstract: The beet-cyst nematode (Heterodera schachtii Schmidt) is one of the major pests of sugar beet. The identification of molecular markers associated with nematode resistance would be helpful for developing resistant varieties. The aim of this study was the identification of SNP (Single Nucleotide Polymorphism) markers for the nematode resistance from the resistant source, WB242. A WB242-F2 population was phenotyped for nematode resistance. DNA of the 4 most resistant and 4 most susceptible individuals was pooled and subjected to Restriction-site Associated DNA Sequencing (RAD-Seq), which generated 7,241 single nucleotide polymorphisms (SNPs). Then, bulked segregant analysis (BSA) was performed by genotyping a subset of 384 SNPs on the 20 most-resistant and the 20 most-susceptible individuals. Among the investigated SNPs, the SNP_192 showed a complete association with nematode resistance. This association was further validated on a set of resistant and susceptible varieties. We conclude that SNP_192 is a valuable marker for high-throughput marker-assisted breeding of nematode resistance in sugar beet.

Last Modified: 10/25/2014
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