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United States Department of Agriculture

Agricultural Research Service

Research Project: Enhancement of Cotton Genetics and Management Systems to Improve Yield, Fiber Quality, and Production Stability

Location: Coastal Plain Soil, Water and Plant Conservation Research

Title: Using RNA-seq transcriptome profiling in support of field based strategies to improve cotton productivity under water deficit stress

Authors
item Campbell, Benjamin
item Bowman, Megan -
item Park, Wonkeun -
item Bauer, Philip
item Scheffler, Brian
item Udall, Joshua -
item Jones, Don -

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: February 3, 2014
Publication Date: February 3, 2014
Citation: Campbell, B.T., Bowman, M., Park, W., Bauer, P.J., Scheffler, B.E., Udall, J., Jones, D.C. 2014. Using RNA-seq transcriptome profiling in support of field based strategies to improve cotton productivity under water deficit stress. In: Proceeedings of the Plant and Animal Genome XXII Conference, January 10-15, San Diego, CA. p918.

Technical Abstract: In the face of changing climatic conditions, water deficit stress is one of the most challenging agricultural issues limiting sustainable cotton production. In this research, our objective was to construct a transcriptome profile of cotton under field water deficit stress. Using RNA-seq and the newly published diploid D5 Gossypium raimondii sequence, we constructed a profile of genes expressed in tetraploid cotton after being exposed to water deficit stress during flowering. Using innovative sequence analysis techniques, transcripts were putatively localized to AT or DT genomes. This study represents one of the first to use RNA-seq techniques and whole genome sequence resources for tetraploid cotton transcriptomics. These data will facilitate efforts to understand the complex responses governing transcriptomic regulatory mechanisms and to identify candidate genes that may benefit applied plant breeding programs.

Last Modified: 7/22/2014
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