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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Improvement of Barley

Location: Cereal Crops Research

Title: Testing transgenic spring wheat and barley lines for reaction to Fusarium head blight: 2013 Field nursery report

Authors
item Koeritz, E -
item Elakkad, A -
item Muehlbauer, G -
item Li, X -
item DAHLEEN, LYNN
item Abebe, T -
item Skadsen, Ronald
item Dill-Macky, R -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 7, 2013
Publication Date: December 3, 2013
Citation: Koeritz, E.J., Elakkad, A.M., Muehlbauer, G.J., Li, X., Dahleen, L.S., Abebe, T., Skadsen, R.W., Dill-Macky, R. 2013. Testing transgenic spring wheat and barley lines for reaction to Fusarium head blight: 2013 Field nursery report. In: Canty, S., Clark, A., Salat, Y., Van Sanford, D., editors. Proceedings of the National Fusarium Head Blight Forum, East Lansing, MI/Lexington, KY: U.S. Wheat and Scab Initiative. National Fusarium Head Blight Forum, December 3-5, 2013, Milwaukee, WI. p. 70.

Technical Abstract: The 2013 field screening nursery consisted of 22 wheat and 15 barley entries evaluated in side by side experiments. Entries within each species experiment were arranged in a randomized complete block design with four replications in a field located at UMore Park, Rosemount MN. Trial entries and untransformed controls* were submitted by the University of Minnesota (16 wheat lines + Bobwhite* and CB037*), and the USDA (10 barley lines + Conlon* and Golden Promise*). Lines with known reactions to Fusarium head blight (FHB) were also included as checks. The wheat checks included were the moderately resistant cultivars Alsen, Rollag and Sumai 3 and the susceptible cultivar Wheaton. The barley checks were the moderately resistant cultivar Quest and the susceptible cultivars, Robust and Stander. Individual plots were 2.43 m long single rows. The trial was planted on June 3, 2013. All plots were inoculated twice. The first inoculation was applied at anthesis for wheat (July 16-Aug 2) and at head emergence (July 19-Aug 2) for barley. The second inoculation was applied three days after the initial inoculation (d.a.i.) for each plot. The inoculum was a composite of 30 F. graminearum isolates at a concentration of 100,000 macroconidia.ml-1 with Tween 20 (polysorbate) added at 2.5 ml.L-1 as a wetting agent. The inoculum was applied using a CO2-powered backpack sprayer fitted with a SS8003 TeeJet spray nozzle with an output of 10 ml.sec-1 at a working pressure of 275 kPa. Mist-irrigation was applied from the first inoculation on July 16 through August 12 to facilitate FHB development. FHB incidence and severity were assessed visually 21 d.a.i. for wheat and around 18 d.a.i. for barley on 20 arbitrarily selected heads per plot. FHB incidence was determined by the percentage of spikes with visually symptomatic spikelets of the 20 heads observed. FHB severity was determined as the percentage symptomatic spikelets of the total of all spikelets observed. Plots were hand harvested at maturity on August 27 (barley) and August 21 (wheat). Fifty heads where harvested from each plot, threshed and the seed cleaned manually. The wheat grain was used to determine the percentage of visually scabby kernels (VSK) and then all samples (wheat and barley) were ground and submitted for deoxynivalenol (DON) analysis. In 2013 the disease severities were generally a little higher than in the 2012 nursery. Mean FHB severities for the untransformed wheat checks, Bobwhite and CB037 were 27 and 17 %, respectively. Mean FHB severities for the other standard wheat checks, Alsen, Wheaton, Rollag and Sumai 3, were 11, 33, 7 and 3%, respectively. For barley, the untransformed check variety Conlon had a mean FHB severity of 15%. The untransformed check Golden Promise and one transformant in the Golden Promise background, were very late heading and did not produce seed. The barley standard checks, Quest, Robust, and Stander had mean FHB severities of 2, 16 and 24%, respectively. For the wheat entries in the Bobwhite background, the FHB severity data indicated that resistance was significantly expressed (P<0.05) in all transformed lines compared to the untransformed Bobwhite check, with all transformed lines similar to Sumai 3 in response. For the entries with a CB037 background, resistance (FHB severity) was significantly (P<0.05) expressed in one transformed line compared to the untransformed background. The FHB severities of all the barley entries in the Conlon background were statistically similar to the untransformed Conlon check, which ranked between Quest and Stander. We would like to acknowledge Beheshteh Zargaran and Julia Hagenguth for their assistance in completion of the work reported. We would also like to acknowledge Dr. Yanhong Dong for conducting the mycotoxin analysis. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No. 59-0206-9-069. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative.

Last Modified: 8/27/2014
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