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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Functional Foods Research » Research » Publications at this Location » Publication #300276
Title: Collision-induced fragmentation accurate mass spectrometric analysis methods to rapidly characterize phytochemicals in plant extracts

Author
item Berhow, Mark
item Duval, Sandra
item Bowman, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/7/2014
Publication Date: 4/9/2014
Citation: Berhow, M.A., Duval, S.M., Bowman, M.J. 2014. Collision-induced fragmentation accurate mass spectrometric analysis methods to rapidly characterize phytochemicals in plant extracts [abstract]. Conference of Food Engineering.

Interpretive Summary:

Technical Abstract: The rapid advances in analytical chromatography equipment have made the reliable and reproducible measurement of a wide range of plant chemical components possible. Full chemical characterization of a given plant material is possible with the new mass spectrometers currently available. New methods are being developed to enhance this analysis to utilize advances in software capability and database availability. For phytochemicals, we have used the latest accurate mass LC-MS analysis to quickly characterize the potential chemical formulas for a series of unknown compounds in a variety of seed and leaf extracts using a standardized CID/HCD mass fragmentation experiment in which interpretation of daughter ions produced allowed for the identification of a variety of phytochemical aglycones and their various substituents. These results – coupled with DEPT NMR analysis as needed – resulted in the identification of a series of phytochemicals in camelina and sorghum extracts. Accurate LC-MSN screening and characterization analyses can be coupled to more rapid non-destructive spectrophotometric analytical methods such as pulsed NMR and near infrared (NIR) spectrometry, which will allow for the rapid and non-destructive analysis of thousands of samples for a wide range of chemical composition parameters.