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United States Department of Agriculture

Agricultural Research Service

Research Project: Genomics and Bioinformatics Research in Agriculturally Important Organisms

Location: Genomics and Bioinformatics Research Unit

Title: Complete genome sequence of channel catfish gastrointestinal sepicemia isolate Edwardsiella tarda C07-087

Authors
item Tekedar, Hasan -
item Karsi, Attila -
item Williams, Michele -
item Vamenta, Stefanie -
item Banes, Michelle -
item Duke, Mary
item Scheffler, Brian
item Lawrence, Mark -

Submitted to: American Society for Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 14, 2013
Publication Date: November 21, 2013
Repository URL: http://handle.nal.usda.gov/10113/58875
Citation: Tekedar, H.C., Karsi, A., Williams, M.L., Vamenta, S., Banes, M.M., Duke, M.V., Scheffler, B.E., Lawrence, M.L. 2013. Complete genome sequence of channel catfish gastrointestinal sepicemia isolate Edwardsiella tarda C07-087. American Society for Microbiology. 1(6):e00959-13. doi:10.1128/genomeA.00959-13.

Interpretive Summary: Edwardsiella tarda is bacterial pathogen that causes edwardsiellosis in fish and other species. E. tarda can cause the disease in both humans and fish usually by starting within the gut, which, if left untreated, can be fatal. However such serious infections in humans are rare. Edwardsiellosis is known in several important aquaculture fish including catfish, tilapia, carp, seabream and flounder. In the past, E. tarda caused minor infections but in recent years sever outbreaks impacting gut functions have occurred. In this paper an isolate of E. tarda was isolated from an outbreak in a Mississippi catfish pond. The bacteria was purified and its DNA sequenced determined by using high throughput DNA sequencing. This isolate and DNA sequence will be used as a reference to study other isolates of E. tarda with the goal of determining the change of E. tarda pathogenicity over time.

Technical Abstract: Edwardsiella tarda is the etiologic agent of acute to chronic edwardsiellosis in fish and other species (1). It is a gram-negative facultative anaerobe that is motile by peritrichous flagella. Edwardsiellosis is an important fish disease that negatively impacts aquaculture industries throughout the world (2). Moreover, E. tarda can cause disease in humans, where it is typically associated with gastrointestinal infections that may progress to haemorrhagic septicemia (3). Historically, E. tarda causes a sporadic disease in channel catfish (Ictalurus punctatus) called emphysematous putrefactive disease, which may begin as small punctate cutaneous erosions that progress to abscesses deep within the musculature (1). However, E. tarda is being increasingly isolated from large outbreaks of gastrointestinal septicemia in commercial channel catfish operations. E. tarda isolate C07-087 is from an outbreak of gastrointestinal septicemia in a commercial catfish pond in Mississippi.

Last Modified: 10/30/2014
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