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United States Department of Agriculture

Agricultural Research Service

Research Project: Plant and Microbial Genetic Resource Preservation and Quality Assessment

Location: Plant And Animal Genetic Resources Preservation Research Unit

Title: Cassava tissue culture and long-term preservation

Authors
item Rodrigues Zebral A., Leonardo -
item Alves, Alfredo -
item Paiva Vilela, Luciano -
item Paiva, Renato -
item Jenderek, Maria
item Ellis, David -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 16, 2013
Publication Date: August 11, 2013
Citation: Rodrigues Zebral A., L., Alves, A.A., Paiva Vilela, L., Paiva, R., Jenderek, M.M., Ellis, D. 2013. Cassava tissue culture and long-term preservation. Meeting Abstract. 2nd International Symposium on Plant Cryopreservation, Fort Collins, CO, Aug 11-14, 2013. pp.102.

Technical Abstract: Cassava (Manihot esculenta Crantz) is cultivated mainly for its starchy roots as an important staple food for the tropics. M. esculenta is the only cultivated species in the genus Manihot, which contains 98 species, mostly native to Brazil. In recent years several research groups have reported methods for cassava tissue culture, offering important applications for germplasm collections, such as conservation, sharing, micropropagation, diseases eradication, among others. However, as in many cultures, in vitro techniques and cryopreservation protocols for cassava are genotype-dependent. Thus, one objective of this study was to extend these investigations. Assessing the factors associated with in vitro regeneration of two cultivars of cassava. Uninodal explants of cassava plants growing in vitro were cultured to obtain elongated axillary shoots. Meristems were excised and cultured in four different culture media. Regeneration, rooting and callus formation were evaluated. A significant difference was found among the tested media. For both cultivars the best culture medium was supplemented with 0.2 mg.L-1 kinetin and 1.0 g.L-1 activated charcoal. As a result of this, various methods of cryopreservation were tested for the development of a cryopreservation protocol for efficient storage of such cultivars. Using the glazing technique, with a solution of modified pre-treatment with 0.5% Tween-20 reached a regeneration rate higher than 50% after exposure to liquid nitrogen.

Last Modified: 12/21/2014
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