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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Improvement of Barley and Oats for Enhanced Quality and Biotic Stress Resistance

Location: Small Grains and Potato Germplasm Research

Title: Registration of the barley transposon-tagged population I:seventy lines each with a single, unique site of Ds insertion

Authors
item Brown, Ryan -
item Dahleen, Lynn
item Lemaux, Peggy -
item Bregitzer, Phil

Submitted to: Journal of Plant Registrations
Publication Type: Germplasm Registration
Publication Acceptance Date: December 5, 2013
Publication Date: May 2, 2014
Citation: Brown, R., Dahleen, L.S., Lemaux, P.G., Bregitzer, P.P. 2014. Registration of the barley transposon-tagged population I:seventy lines each with a single, unique site of Ds insertion. Journal of Plant Registrations. 8:226-230.

Interpretive Summary: The Agricultural Research Service, U.S. Dept. of Agriculture (USDA-ARS), has developed and released a new genetic stock resource, a transposon-tagging barley (Hordeum vulgare L.) population comprised of 70 lines. Each line contains a single Ds-bar transposable element insertion at a different genomic location. Transposable elements are mobile pieces of DNA, and when they move into a gene, they will disrupt the function of the gene, a mutation will result. By studying the effects on the plant, and the genetic sequence of the gene that has been mutated, the function and characteristics of the gene can be determined. By studying gene function, the role of each gene in regulatory or metabolic pathways can be determined and assist in the understanding of how plants develop and produce useful products, such as grain for food and feed products. Therefore it is expected that these lines will be useful for the study of barley growth and development.

Technical Abstract: The Agricultural Research Service, U.S. Dept. of Agriculture (USDA-ARS), has developed and released a new genetic stock resource, a transposon-tagging barley (Hordeum vulgare L.) population comprised of 70 lines, TNP 200-TNP 207, TNP 210—TNP 229, TNP 232, TNP 233, TNP 235, TNP 237—TNP 262, TNP 264—TNP 267, TNP 269, and TNP 273—TNP 280 (Reg. No., xxxxx–xxxxx; PIs xxxxx–xxxxxx). These designations are identical to the designations used during line development. Each line contains a single Ds-bar transposable element insertion at a different genomic location and in a homozygous state. The ~3.6 kb Ds-bar element is a modified maize Dissociation (Ds) element containing a bar expression cassette. These lines were derived from primary Ds (PDS) transpositions resulting from biolistic co-introduction of Ds-bar and maize AcTransposase (AcT) into ‘Golden Promise’ (PI 343079). Lines containing secondary, tertiary, and quaternary transpositions were generated by remobilizing Ds-bar via sexual hybridization of transgenic Golden Promise plants expressing AcT plants with PDS lines and with derived TNP lines containing secondary and tertiary transpositions, respectively. Each line has been characterized for the 5’ and/or 3’ sequences flanking the Ds-bar insertion. Insertions that interrupt native Golden Promise DNA genes or regulatory elements likely will alter or abolish their function. Therefore it is expected that these lines will be useful for the study of the biological roles of such genes or regulatory sequences. These lines can also be used as parents for the creation of additional lines with novel transpositions of Ds-bar.

Last Modified: 12/21/2014
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