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United States Department of Agriculture

Agricultural Research Service

Research Project: Improving Postharvest Life of Potted Plants and Cut Flowers through Use of Molecular and Applied Technologies

Location: Crops Pathology and Genetics Research

Title: Transcriptome changes associated wtih delayed flower senescence on transgenic petunia by inducing expression of etr1-1, a mutant ethylene receptor

Authors
item Wang, Hong -
item Stier, Genevieve -
item Lin, Jing -
item Liu, Gang -
item Zhang, Zhen -
item Chang, Youhong -
item Reid, Michael -
item Jiang, Cai-Zhong

Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 26, 2013
Publication Date: July 9, 2013
Repository URL: http://link.springer.com/article/10.1007%2Fs13197-013-0986-y#page-1
Citation: Wang, H., Stier, G., Lin, J., Liu, G., Zhang, Z., Chang, Y., Reid, M.S., Jiang, C. 2013. Transcriptome changes associated wtih delayed flower senescence on transgenic petunia by inducing expression of etr1-1, a mutant ethylene receptor. PLoS One. 8(7):1-14.

Technical Abstract: Flowers of ethylene-sensitive ornamental plants transformed with ethylene-insensitive 1-1(etr 1-1), a mutant ethylene receptor first isolated from Arabidopsis, are known to have longer shelf lives. We have generated petunia plants in which the etr 1-1 gene was over-expressed under the control of a chemically-inducible promoter, which would allow expression of etr 1-1 to be initiated at the desired time and stage of development. Here, we showed that transgenic plants grew and developed normally without a chemical inducer. Semi-quantitative RT-PCR demonstrated that the abundance of transcripts of Arabidopsis etr 1-1 gene was substantially induced in flowers with 30 uM dexamethasone (DEX). Consequently, the life of the flowers was almost doubled and the peak of ethylene production was delayed. We compared gene expression changes of petals with DEX to those without DEX at 24 h and 48 h by microarray. Our results indicated that transcripts of many putative genes encoding transcription factors were down-regulated by etr 1-1 induced expression at the early stage. In addition, putative genes involved in gibberellin biosynthesis, response to jasmonic acid/gibberellin stimulus, cell wall modification, ethylene biosynthesis, and cell death were down-regulated associating with etr 1-1 induced expression. We investigated time-course gene expression profiles and found two profiles which displayed totally opposite expression patterns under these two treatments. In these profiles, 'the regulation of transcription' was predominant in GO categories. Taking all results together, we concluded those transcription factors down-regulated at early stage might exert a major role in regulating the senescence process which were consequently characterized by cell wall modification and cell death.

Last Modified: 7/24/2014
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