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United States Department of Agriculture

Agricultural Research Service

Research Project: PRODUCTION, STABILIZATION AND FORMULATION OF MICROBIAL AGENTS AND THEIR NATURAL PRODUCTS

Location: Crop Bioprotection Research

Title: Draconibacterium orientalis gen. nov. sp. nov.

Authors
item Du, Zong-Jun -
item Wang, Ying -
item Dunlap, Christopher
item Rooney, Alejandro
item Chen, Guan-Jun -

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 4, 2014
Publication Date: May 12, 2014
Citation: Du, Z., Wang, Y., Dunlap, C.A., Rooney, A.P., Chen, G. 2014. Draconibacterium orientalis gen. nov. sp. nov. International Journal of Systematic and Evolutionary Microbiology. 64(5):1690-1696.

Interpretive Summary: This manuscript describes a new genus and species of bacteria, Draconibacterium orientalis, isolated from the Yellow Sea. This new species also represents a new taxonomic Family, Class, and Order of bacteria previously unknown to science. Bacteria from the marine environment possess unique and sometimes unusual biochemical characteristics that make them attractive candidates to explore their potential for new uses in agriculture or biotechnology. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for biological control applications can be assessed in future studies.

Technical Abstract: The taxonomic characteristics of two bacterial strains, FH5T and SS4, isolated from enrichment cultures obtained from two distinct marine environments, were determined. These bacteria were Gram-stain-negative, facultatively anaerobic rods. The temperature range for growth was 20-40 °C and the pH for growth ranged from 5.5 to 9.0, with optimum growth occurring in the temperature range 28-32 °C and pH range 7.0-7.5. Growth occurred in the presence of 1-7 % (w/v) NaCl, with optimum growth occurring in the presence of 2-4 % (w/v) NaCl. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0. MK-7 is the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine (PE), an unkown phospholipid (PL1) and an unknown lipid (L2). The DNA G+C contents of strains FH5T and SS4 were both determined to be 42.0%. The results of DNA-DNA hybridization studies indicated that the FH5T and SS4 genomes are highly similar (greater than 95% similarity). The strains formed a distinct phyletic line within the class Bacteroidia, with less than 89.4% sequence identity to their closest relatives with validly published names. On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, Draconibacterium orientalis gen. nov., sp. nov., within the class Bacteroidia are proposed, with strain FH5T (=DSM 25947T=CICC 10585T) as the type strain of the genus. In addition, a new family name, Draconibacteriaceae fam. nov., is proposed to accommodate Draconibacterium gen. nov. Here, Dracobacteriales ord. nov. is proposed and we suggest to transfer family Marinilabiliaceae to the order Dracobacteriales as Marinilabiliaceae comb. nov.

Last Modified: 7/25/2014
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