Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Improvement of Small Grains for Biotic and Abiotic Stress Tolerance and Characterization of Pathogen Populations

Location: Plant Science Research

Title: Three-dimensional reconstruction of frozen and thawed plant tissues from microscopic images

Authors
item Livingston, David
item Tuong, Tan Duy

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: August 15, 2013
Publication Date: March 1, 2014
Citation: Livingston, D.P., Tuong, T.D. 2014. Three-dimensional reconstruction of frozen and thawed plant tissues from microscopic images. Book Chapter. 1166:117-137.

Interpretive Summary: 3D reconstruction of plant tissues using medical imaging techniques has been impossible due to the need for higher resolution to see internal structures of plants. In this book of Methods used in Cold Acclimation we present a technique we developed to generate 3D images of plants from conventional histological sections that have been stained to reveal internal structures. A complete and detailed tutorial is presented using the commercially available software Adobe After Effects. The technique is also suited for medical histological samples that may be too small for MRI or CT but too big for Confocal Microscopy.

Technical Abstract: Histological analysis of frozen and thawed plants has been conducted for many years but the observation of individual sections only provides a 2 dimensional representation of a 3 dimensional phenomenon. Most techniques for viewing internal plant structure in 3 dimensions is either low in resolution or the instrument cannot penetrate deep enough into the tissue to visualize the whole plant. Techniques with higher resolution are expensive and equipment often requires time-consuming training. We present a relatively simple and less expensive technique using pixel-based (JPEG) images of histological sections of an Arabidopsis plant and commercially available software to generate 3D reconstructions of internal structures. The technique has proven to work just as effectively for images from medical histology.

Last Modified: 10/22/2014
Footer Content Back to Top of Page