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United States Department of Agriculture

Agricultural Research Service

Research Project: Management of Temperate-Adapted Fruit, Nut, and Specialty Crop Genetic Resources and Associated Information

Location: National Clonal Germplasm Repository (Corvallis, Oregon)

Title: Antioxidants and cryopreservation, the new normal?

Author
item Reed, Barbara

Submitted to: Acta Horticulturae
Publication Type: Abstract Only
Publication Acceptance Date: June 5, 2013
Publication Date: August 11, 2013
Citation: Reed, B.M. 2013. Antioxidants and cryopreservation, the new normal? [Abstract] Acta Horticulturae. Pg. 18.

Interpretive Summary: Cryopreservation protocols are established for many plant species. Cryopreservation provides stable, long-term and low-cost backups that are safe from the diseases or environmental damage that challenge whole plants. However, some plants respond poorly to cryopreservation due to osmotic stress or lack of tolerance to low temperatures. Various stresses can damage cell components. Plants have evolved natural antioxidant defense mechanisms to combat these effects. In addition to temperature-induced stresses, cryopreservation protocols have osmotic and chemical effects on plant cells that contribute to the vitrification process, but also increase cellular oxidation. Cryopreservation protocols that include antioxidants resulted in increased regrowth of plant tissues after warming.

Technical Abstract: Cryopreservation protocols are established for many plant species. Cryopreservation provides stable, long-term and low-cost backups that are safe from the diseases or environmental damage that challenge whole plants. However, some plants respond poorly to cryopreservation due to osmotic stress or lack of tolerance to low temperatures. Various stresses can increase reactive oxygen species (ROS) formation to toxic levels in cells and tissues. ROS include superoxide radicals, hydroxyl radicals, hydrogen peroxide and singlet oxygen. Plants have evolved natural antioxidant defense mechanisms to combat the effects of ROS that are produced during physiological stress. These ROS scavenging mechanisms include antioxidant enzymes such as superoxide dismutase, peroxidases, mono- and dehydroascorbate reductase, glutathione reductase and catalase. In addition to temperature-induced stresses, cryopreservation protocols have osmotic and chemical effects on plant cells that contribute to the vitrification process, but also increase cellular oxidation. Cryopreservation protocols that include antioxidants during the cryopreservation process resulted in reduced oxidation and increased regrowth of plant tissues.

Last Modified: 9/20/2014
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