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United States Department of Agriculture

Agricultural Research Service

Research Project: CEREAL RUST FUNGI: GENETICS, POPULATION BIOLOGY, AND HOST-PATHOGEN INTERACTIONS

Location: Cereal Disease Laboratory

Title: Identification of wheat gene Sr35 that confers resistance to Ug99 stem rust race group

Authors
item Saintenac, Cyrille -
item Zhang, Wenjun -
item Salcedo, Andres -
item Rouse, Matthew
item Trick, Harold -
item Akhunov, Eduard -
item Dubcovsky, Jorge -

Submitted to: Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 10, 2013
Publication Date: June 27, 2013
Citation: Saintenac, C., Zhang, W., Salcedo, A., Rouse, M.N., Trick, H., Akhunov, E., Dubcovsky, J. 2013. Identification of wheat gene Sr35 that confers resistance to Ug99 stem rust race group. Science [online]:1239022. http://www.sciencemag.org/content/early/2013/06/26/science.1239022.

Interpretive Summary: Wheat stem rust is a devastating disease that can cause severe yield losses in wheat. A new race of the wheat stem rust fungal pathogen designated Ug99 has overcome most of the widely used resistance genes and is spreading through Africa and Asia threatening major wheat production areas. We report here the cloning of the Sr35 gene from a wild wheat relative, which confers resistance to Ug99. Using map-based cloning, association mapping, induced mutations, and transgenic complementation we demonstrate that Sr35 is a CC-NBS-LRR gene designated CNL9. This gene is absent in wheat and in modern cultivars, but is effective when transferred to bread wheat. The cloning of Sr35 opens the door to the use of biotechnological approaches to control this devastating disease, and to the analyses of the molecular interactions that define the wheat-rust pathosystem. The products of this research will be used by scientists to plan further experiments to develop new technologies to protect wheat from yield losses to rust diseases.

Technical Abstract: Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt) is a devastating disease that can cause severe yield losses. A new Pgt race designated Ug99 has overcome most of the widely used resistance genes and is spreading through Africa and Asia threatening major wheat production areas. We report here the cloning of the Sr35 gene from Triticum monococcum, which confers resistance to Ug99 and related Pgt races. Using map-based cloning, association mapping, induced mutations, and transgenic complementation we demonstrate that Sr35 is a CC-NBS-LRR gene designated CNL9. This gene is absent in the diploid donor of the A genome of polyploid wheat and in modern cultivars, but is effective when transferred to bread wheat. The cloning of Sr35 opens the door to the use of biotechnological approaches to control this devastating disease, and to the analyses of the molecular interactions that define the wheat-rust pathosystem.

Last Modified: 12/17/2014
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