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United States Department of Agriculture

Agricultural Research Service

Research Project: SYSTEMATICS, GENETIC DIVERSITY ASSESSMENT, AND ACQUISITION OF POTATOES, CARROTS, AND THEIR RELATED WILD RELATIVES

Location: Vegetable Crops Research Unit

Title: Genetic structure and domestication of carrot (Daucus carota subsp. sativus L.) (Apiaceae)

Authors
item Iorizzo, Massimo -
item Senalik, Douglas
item Ellison, Shelby -
item Grzebelus, Dariusz -
item Cavagnaro, Pablo -
item Allender, Charlotte -
item Brunet, Johanne
item Spooner, David
item Van Deynze, Allen -
item Simon, Philipp

Submitted to: Botanical Society of America Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: April 30, 2013
Publication Date: July 28, 2013
Citation: Iorizzo, M., Senalik, D.A., Ellison, S.L., Grzebelus, D., Cavagnaro, P.F., Allender, C., Brunet, J., Spooner, D.M., Van Deynze, A., Simon, P.W. 2013. Genetic structure and domestication of carrot (Daucus carota subsp. sativus L.) (Apiaceae) [abstract]. Botanical Society of America Abstracts. Paper No. 654.

Technical Abstract: Analyses of genetic structure and phylogenetic relationships illuminate the origin and domestication of modern crops. Despite being an important world-wide vegetable, the genetic structure and domestication of carrot (Daucus carota L.) is poorly understood. We provide the first such study using a large data set of molecular markers and accessions, widely dispersed around the world. Sequencing data from the carrot transcriptome were used to develop 4000 Single Nucleotide Polymorphisms (SNP). Eighty-four genotypes, including a geographically well-distributed subset of wild and cultivated carrots, were genotyped using the KASPar assay. Analysis of allelic diversity of SNP data revealed no reduction of genetic diversity in cultivated vs. wild accessions. Structure and phylogenetic analysis indicated a clear separation between wild and cultivated accessions as well as between eastern and western cultivated carrot. Among the wild carrots, those from Central Asia were genetically most similar to cultivated accessions. Furthermore, we found that wild carrots from North America were most closely related to European wild accessions. Comparative genetic diversity of wild and cultivated accessions suggested the absence of a genetic bottleneck during carrot domestication. In conjunction with historical documents, our results suggest an origin of domesticated carrot in Central Asia. Wild carrots from North America were likely introduced as weeds with European colonization. These results provide answers to long-debated questions of carrot evolution and domestication, and inform germplasm curators and breeders on genetic substructure of carrot genetic resources.

Last Modified: 4/21/2014
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