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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetics, Physiology, and Health Research to Improve Catfish Production

Location: Warmwater Aquaculture Research Unit

Title: Comprehensive survey and genomic characterization of toll-like receptors (TLRS) in channel catfish, Ictalurus punctatus: Identification of novel fish TLRS

Authors
item Quiniou, Sylvie
item Boudinot, Pierre -
item Bengten, Eva -

Submitted to: Fish and Shellfish Immunology
Publication Type: Abstract Only
Publication Acceptance Date: April 6, 2013
Publication Date: June 25, 2013
Citation: Quiniou, S., Boudinot, P., Bengten, E. 2013. Comprehensive survey and genomic characterization of toll-like receptors (TLRS) in channel catfish, Ictalurus punctatus: Identification of novel fish TLRS. Fish and Shellfish Immunology. P.313.

Technical Abstract: A comprehensive survey of channel catfish Toll-Like Receptors (TLRs) was undertaken following a genomic PCR approach based on degenerate primers. Twenty different TLRs were identified in channel catfish. Channel catfish TLR sequences were characterized by phylogenetic analysis based on their conserved Toll/Interleukin-1 Receptor (TIR) domain and by in depth analysis of Leucine Rich Repeat (LRR) motifs of the ligand binding Extra Cellular Domain (ECD). The catfish have representatives of all the TLR types defined in vertebrates with the exception of 6, 10, 11, 12, 13, 15, 23 and 24. Additionally two new types were discovered TLR 25 and TLR 26. TLR25 is also present in cyprinids, cichlids, plecoglossids, and adrianichthyids suggesting its presence early in fish evolution. To date, TLR26 was found only in channel catfish. Like TLR18-23, TLR 25 and 26 were not found in any other vertebrate classes and appear to be fish specific. Data mining using the catfish TLR sequences revealed that in addition to ictalurids and cyprinids TLR4 is also present in salmonids. TLR19 and TLR20 were both found in ictalurids, cyprinids and salmonids, demonstrating a wider range than previously known. The LRR structure within ECDs appeared generally well conserved. TLR 7 demonstrated a very high identity to human TLR7 strongly suggesting that ligand specificity maybe conserved. Finally, expression profiling confirmed that most TLR are widely expressed in a diversity of tissues, and revealed marked differences of expression level.

Last Modified: 10/1/2014
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