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United States Department of Agriculture

Agricultural Research Service

Research Project: UTILIZATION OF GENOMICS FOR IMPROVING PRODUCTION TRAITS IN COOL AND COLD WATER AQUACULTURE

Location: Cool and Cold Water Aquaculture Research

Title: Effect of sexual maturation on muscle gene expression of rainbow trout on a high nutritional plane: RNA-Seq approach

Authors
item Salem, Mohamed -
item Manor, Meghan -
item Aussanasuwannakul, Aunchalee -
item Kenney, P. Brett -
item Weber, Gregory
item Slider, Susan -
item Yao, Jianbo -

Submitted to: Aquaculture Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 16, 2012
Publication Date: February 24, 2013
Citation: Salem, M., Manor, M.L., Aussanasuwannakul, A., Kenney, P., Weber, G.M., Slider, S.D., Yao, J. 2013. Effect of sexual maturation on muscle gene expression of rainbow trout on a high nutritional plane: RNA-Seq approach. Aquaculture Conference Proceedings. February 22-25, 2013, Nashville, Tennessee. p. 912.

Technical Abstract: Muscle degradation occurs as a response to various physiological and pathological conditions regulated by specific molecular mechanisms. Previously, we characterized the physiological and metabolic changes of muscle deterioration associated with the female sexual maturation in rainbow trout. Most of the muscle deterioration signs of this model represent nutrient mobilization as a response to the energetic over-demands of the egg/ovarian growth phase. In this study, we investigated effects of sexual maturation on the muscle of rainbow trout fed on a high plane of nutrition. The elevated nutritional plane negated most of the muscle deterioration that would have been associated with the nutrient demands of vitellogenesis, including reductions in muscle mass and protein content, previously characterized when animals were raised under ordinary farm feed rations. Gravid fish showed reduced total fat, relative saturated and monounsaturated fatty acids and elevated polyunsaturated fatty acids compared to sterile fish. The RNA-Seq technique identified gene expression signature that is consistent with metabolic changes of the gravid fish during spawning season (December and January) compared to sterile fish. Gravid fish showed increased abundance of transcripts in the metabolic pathways of fatty acid degradation and up- and down-regulated expression of genes involved in biosynthesis and degradation, of the polyunsaturated fatty acids, respectively. In addition, gravid fish showed increased expression of genes involved in citrate cycle and oxidative phosphorilation. The muscle transcriptomic signature of fish fed a high nutritional plane is quite distinct from that described under low nutritional plane. The study points out that enhancement of nutrition during sexual maturation can recover many of the adverse effects on fish muscle growth and fillet quality.

Last Modified: 8/30/2014