Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 25, 2013
Publication Date: April 19, 2013
Citation: Miller, L.C., Jiang, Z., Sang, Y., Harhay, G.P., Lager, K.M. 2013. Evolutionary characterization of pig interferon-inducible transmembrane gene family and member expression dynamics in tracheobronchial lymph nodes of pigs infected with influenza A virus. Second Annual Great Plains Emerging Infectious Diseases Conference. p. 23. Technical Abstract: Studies have found that a cluster of duplicated gene loci encoding the interferon-inducible transmembrane proteins (IFITMs) family have antiviral activity against several viruses, including influenza A virus (IAV). Whether the duplicated members have selective viral targets, recognition patterns and signaling pathways against different influenza subtypes remain largely unexplored. Interestingly, the porcine IFITM family is quite diversified, having at least 12 potentially functional gene loci, while humans and mice possess only 5 and 7 members, respectively. Phylogenic analyses showed porcine IFITM genes have a positive selection over IFITM1 paralogs, and constitute seven members, which have highly conserved human/mouse orthologs that exert different anti-IAV activity. In this context, we identified and compared gene expression changes in swine tracheobronchial lymph nodes following viral infection using Digital Gene Expression Tag Profiling (DGETP). Our transcriptome analysis demonstrated multiple IFITM genes were differentially expressed over 14 days post-inoculation by a swine IAV.