Title: Montanide ISA 71 VG adjuvant enhances antibody and cell-mediated immune responses to profilin subunit antigen vaccination and promotes protection against Eimeria acervulina and Eimeria tenella Authors
Submitted to: Experimental Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 20, 2012
Publication Date: December 16, 2012
Citation: Jang, S.I., Lillehoj, H.S., Lee, S.H., Lee, K.W., Lillehoj, E., Bertrand, F., Dupuis, L., Deville, S. 2012. Montanide ISA 71 VG adjuvant enhances antibody and cell-mediated immune responses to profilin subunit antigen vaccination and promotes protection against Eimeria acervulina and Eimeria tenella. Experimental Parasitology. 127:178-183. Interpretive Summary: Coccidiosis is caused by several distinct species of Eimeria protozoan parasites which infect different areas of the gut, severely reducing host nutrient absorption. Prophylactic medication and live vaccines are widely used to control coccidiosis. However, with increasing concerns on the rising drug resistant bacteria, and increasing cost to produce live parasite vaccines, the industry needs alternative strategies to control coccidiosis. In this study, ARS scientists collaborated with scientists at a private company to develop a recombinant vaccination strategy to control coccidiosis. This paper describes a novel recombinant vaccine formulation which combines an immunogenic parasite antigen with a commercially available adjuvant to enhance the immunogenicity of the recombinant parasite vaccine. Young broiler chickens which were immunized with this novel vaccine-adjuvant complex showed significantly enhanced protection against a challenge infection with live parasites. These findings provide a possibility of developing an effective peptide vaccine against coccidiosis using an immunogenic parasite protein when it is delivered with an immune stimulating adjuvant. These results may hasten the development of an efficacious vaccine, which could reduce the costs and the demand for antibiotics in poultry production.
Technical Abstract: The present study was conducted to investigate the immunoenhancing effects of MontanideTM ISA 71 VG adjuvant on profilin subunit antigen vaccination. Broiler chickens were immunized subcutaneously with a purified Eimeria acervulina recombinant profilin protein, either alone or mixed with ISA 71 VG, and host immune responses were evaluated. After secondary immunization, antigen-specific antibody and T-cell responses were higher in the group which received profilin plus ISA71 VG compared with the other groups. Furthermore, body weight gains and fecal oocyst shedding were evaluated following oral challenge infection with live E. acervulina or E. tenella oocysts. Vaccination with profilin plus ISA 71 VG reduced oocyst shedding compared with animals immunized with profilin alone. These results demonstrate that the recombinant profilin subunit vaccine, when given in combination with MontanideTM ISA 71 VG, augments protective immunity against E. acervulina and E. tenella.