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United States Department of Agriculture

Agricultural Research Service

Research Project: NOVEL TECHNOLOGY FOR RENEWABLE RESOURCE UTILIZATION

Location: Renewable Product Technology Research Unit

Title: Optimization of culture conditions for production of a novel cold-active lipase from Pichia lynferdii NRRL Y-7723

Authors
item Park, Sun-Young -
item Kim, Ji-Yeon -
item Bae, Jae-Han -
item HOU, CHING
item Kim, Hak-Ryul -

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 10, 2013
Publication Date: January 30, 2013
Citation: Park, S., Kim, J., Bae, J., Hou, C.T., Kim, H. 2013. Optimization of culture conditions for production of a novel cold-active lipase from Pichia lynferdii NRRL Y-7723. Journal of Agricultural and Food Chemistry. 61(4):882-886.

Interpretive Summary: Lipase is one of the most important enzymes used in a broad range of industrial applications. Lipases with that do not function well in areas such as thermo stability, alkaline, acidic, and old-activity receive special attention because of their applicability in the restricted reaction conditions. In this study, we maximized the production of a novel cold-active lipase from P. lynferdii NRRL Y-7723 and found at the optimum yeast extract level the lipase production was over ten times higher than the productivity under standard conditions. All natural oils tested showed lipase production, but their maximum productivities varied according to incubation time and oil species. In addition to lipase application in other industries, the results of this study may also be used in the preparation of functional/health foods for improved health and nutrition.

Technical Abstract: Lipases with abnormal properties such as thermo stability, alkalinity, acidity and cold-activity receive industrial attention because of their usability under restricted reaction conditions. Most microbial cold-active lipases originate from psychrotrophic and psychrophilic microorganisms found in Antarctic regions, which has led to difficulties in the practical production of cold-active lipase. Recently a mesophilic yeast, Pichia lynferdii NRRL Y-7723, was reported to produce a novel cold-active lipase. In this study, we focused on optimization of environmental factors, while giving particular attention to the relationships between given factors and incubation time, to maximize the production of a novel cold-active lipase from P. lynferdii NRRL Y-7723. Maximum lipase production was highly dependent on the incubation time at a given environmental factor. Lipase production varied with incubation time at a given temperature and 20°C was selected as the optimum temperature for lipase production. Fructose was selected as the best carbon source, and maximum lipase production was obtained when it was present at 0.7% (w/v). Yeast extract was an efficient organic nitrogen source, with the maximum lipase production occurring at 0.9% (w/v). Specifically, at the optimum yeast extract level the lipase production was over ten times higher than the productivity under standard conditions. All natural oils tested showed lipase production, but their maximum productivities varied according to incubation time and oil species.

Last Modified: 9/29/2014
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