Technical Abstract: We have developed 384 simple sequence repeat (SSR) markers for the identification of accessions of Chionanthus retusus and four related species. The bark of C. retusus and C. virginicus is used in the industry of natural product to treat inflammation, fever and other illnesses, and with the use of these markers the industry could test for the correct identification of the materials for manufacturing. The use of these markers could also benefit the nursery of ornamentals, allowing for the accurate identification of materials for propagation and crosses. Finally, since Chionanthus is part of the Oleaceae family, together with Olive and Tropical Ash, the availability of the markers we developed could be tested in these species for breeding purposes and ecological studies.