Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: ADVANCED CONVERSION TECHNOLOGIES FOR SUGARS AND BIOFUELS: SUPERIOR FEEDSTOCKS, PRETREATMENTS, INHIBITOR REMOVAL, AND ENZYMES

Location: Bioenergy Research Unit

Title: Phylogeny-guided screening of yeast strains for lipid production

Authors
item Dien, Bruce
item Slininger, Patricia
item Kurtzman, Cletus
item Evangelista, Roque
item Moser, Bryan
item Saha, Badal
item Cotta, Michael
item Balan, V -
item Jin, M -
item Sousa, L -
item Dale, B -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 29, 2013
Publication Date: May 2, 2013
Citation: Dien, B.S., Slininger, P.J., Kurtzman, C.P., Evangelista, R.L., Moser, B.R., Saha, B.C., Cotta, M.A., Balan, V., Jin, M., Sousa, L.D., Dale, B.E. 2013. Phylogeny-guided screening of yeast strains for lipid production [abstract]. Society for Industrial Microbiology and Biotechnology. Poster 8-10.

Technical Abstract: Oleaginous yeast accumulates greater than 20% of their biomass as triacylglycerol in response to nutritional starvation in the presence of excess carbon source. As such, these yeasts have been suggested as a biocatalyst for converting sugars derived from cellulosic feedstocks into biodiesel. Several yeast strains belonging to various oleaginous species were selected from the Agricultural Research Service (ARS) Culture Collection (NCAUR, Peoria, IL) and were screened for lipid production. The oleaginous yeast strains were screened in baffled flask cultures using a complex glucose and/or xylose-based medium with a high C:N ratio. Screening was facilitated by adapting a chemical assay that allows for in situ lipid measurement, avoiding the need for extraction. Two strains, from an initial screen of 12, were found to store over 50% of their biomass as lipids. One of these was selected for further study based upon lipid yield and productivity. The strain was able to readily convert xylose and glucose/xylose mixtures simultaneously to lipids at yields near the practical maximum of circa 0.2 g/g sugar. Related isolates available from the ARS culture collection are now being screened for superior oil-producing ability based upon relatedness using a phylogenetic tree for guidance.

Last Modified: 7/22/2014
Footer Content Back to Top of Page