|Brueggeman, Robert -|
|Abebe, Tilahun -|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 11, 2012
Publication Date: December 4, 2012
Citation: Dahleen, L.S., Brueggeman, R., Abebe, T., Skadsen, R.W. 2012. 2012 North Dakota transgenic barley FHB nursery report. Meeting Abstract. Proceedings of the 2012 National Fusarium Head Blight Forum. p 132. Technical Abstract: The 2012 North Dakota transgenic field trials consisted of 23 barley lines, tested in three misted and three non-misted replicates. Plots were sown on May 9, 2012 in hill plots with 10 seed per hill spaced at 30 cm, and all plots were inoculated using the grain spawn method at heading. Lines included Conlon, the resistant checks Quest and CI4196, twelve primary transgenic lines derived from Conlon, and four transgenic-null pairs derived from crosses between primary Golden Promise transgenics and Conlon. FHB severity was evaluated approximately three weeks after anthesis, by counting the total and infected number of seed on ten randomly selected spikes per row. DON concentrations in the barley samples were determined by gas chromatography with electron capture detection using the method of Tacke and Casper. FHB and DON data were analyzed by SAS (SAS Institute, Cary, NC) with means adjusted for the nearest checks. Average FHB severity was 16% over all six replicates, 9% in the non-irrigated plots and 23% in the irrigated plots. Average DON contamination was 5.1ppm over all plots with 1.9ppm in the non-irrigated plots and 8.5ppm in the irrigated plots. Standard deviations for DON among the replicates were extremely high, reducing the power of comparisons. These lines will be analyzed again in the 2013 transgenic FHB nursery.