Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 2, 2012
Publication Date: April 9, 2013
Citation: Picklo, M.J. 2013. Vitamin E and Vitamin C supplementation does not prevent glucose intolerance in obese-prone rats. Federation of American Societies for Experimental Biology Conference. 27:1067.3. Technical Abstract: Obesity-induced glucose intolerance affects over 70 million Americans. Elevated oxidative stress is associated with development of glucose intolerance. In this work, we tested the hypothesis that supplementation with the anti-oxidants vitamin E (d-alpha-tocopherol acetate; 0.4 g/kg diet) and vitamin C (0.5 g/kg diet) prevents glucose intolerance in obese-prone Sprague-Dawley (OBSD) rats. OBSD rats were fed either a low-fat diet (LF; 10% of calories from lard; n=14), a high-fat diet (HF, 45% of calories from lard; n=14), or a high-fat diet with supplemented vitamin E and vitamin C (HF-CE; n=14). Induction of obesity was characterized by significantly elevated body fat and hyperlipidemia. Anti-oxidant supplementation had no effect upon these parameters. Following 16 weeks on this diet, oral glucose tolerance tests were performed with a dose of glucose 1.5 g/kg per kg lean body mass. While the HF diet caused glucose intolerance, anti-oxidant supplementation did not prevent obesity-induced increases in fasting glucose levels, HF: 75± 6 mg/dL, HF-CE:76 ± 5 mg/dL, and LF:64±8 mg/dL nor prevented obesity-induced increases in the glucose: HF: 13,155 ± 1516 mg*min/dL, HF-CE: 13211 ± 1063 mg*min/dL, and LF 10411 ± 1328 mg*min/dL. These data indicate that vitamin E and vitamin C supplementation does not prevent glucose intolerance. This work was supported by the USDA-ARS.