Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 2, 2012
Publication Date: April 9, 2013
Citation: Whigham Grendell, L.D., Jahns, L.A., Claycombe, K.J., Johnson, L. 2013. Consumption of carotenoid-rich diet improves plasma inflammatory markers. Federation of American Societies for Experimental Biology Conference. 27:846.2. Technical Abstract: Multiple studies have investigated the effects of carotenoids on a limited selection of cytokines. However, inflammation is a complex system involving numerous interacting cytokines. In this study, a broad array of 30 cytokines was measured after consumption of low- and high-carotenoid diets. Nine men and 19 women (age 32.3 ± 13.1 y, BMI 23.9 ± 3.2 kg/m2) consumed their usual diet while excluding high-carotenoids foods (6 wk, depletion) followed by a provided diet based on the Dietary Guidelines (8 wk, repletion; 62 mg carotenoids/d). Plasma was collected after an overnight fast at baseline, end of depletion, and end of repletion. Cytokine concentrations (Milliplex Human Cytokine/Chemokine kits) at each time point were ranked within each subject and analyzed using repeated measures ANOVA followed by Tukey contrasts. Compared to baseline, levels of the pro-inflammatory cytokines tumor necrosis factor (TNF)-', macrophage inflammatory protein (MIP)-1', and MIP-1' were no different after depletion, but levels were decreased after carotenoid repletion (p < 0.05). Interferon-', which can act to inhibit TNF-' secretion, increased after repletion. There was no effect of diet on the other 26 cytokines. These data support an anti-inflammatory role of dietary carotenoids in healthy volunteers. Study supported by USDA-ARS Grand Forks Human Nutrition Research Center.