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United States Department of Agriculture

Agricultural Research Service

Research Project: ALLIUM, CUCUMIS, AND DAUCUS GERMPLASM ENHANCEMENT, GENETICS, AND BIOCHEMISTRY

Location: Vegetable Crops Research Unit

Title: An international plan to sequence the nuclear genome of onion

Author
item HAVEY, MICHAEL

Submitted to: National Allium Research Conference
Publication Type: Abstract Only
Publication Acceptance Date: September 22, 2012
Publication Date: November 29, 2012
Citation: Havey, M.J. 2012. An international plan to sequence the nuclear genome of onion [abstract]. National Allium Research Conference. Paper No. O-23.

Technical Abstract: As large-scale DNA sequencing technologies become more efficient and less costly, the genomic DNAs of more and more plants are being sequenced, assembled, and annotated. These complete sequences are extremely valuable for the identification of specific genes associated with important phenotypes. This presentation will propose an efficient strategy for an international effort to sequence the onion nuclear DNA and provide the sequences and annotations on a freely accessible website. Deep transcriptome sequencing is already underway. Reduced representation approaches can be used to select against repetitive DNAs and enrich for more unique regions of the onion genome. These genomic reads can be aligned against expressed sequences of the transcriptome to identify promoter and intronic regions. The international community should select and focus on one doubled haploid line as the common reference material for collaborative sequencing efforts. A publically accessible website should provide unrestricted access to the sequence and annotations. These resources will enable translational genomics of onion by providing researchers world-wide tools to more efficiently select for important traits in onion improvement.

Last Modified: 7/25/2014