DEVELOPMENT OF HIGH QUALITY, COST-EFFECTIVE, MASS-REARED BIOCONTROL AGENTS FOR SMALL AND URBAN FARMS, ORGANIC FARMS AND GREENHOUSES
Location: Biological Control of Insects Research
Title: The assessment of genetic variability among spined soldier bug (Pentatomidae: Hemiptera) populations
Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: October 25, 2012
Publication Date: October 30, 2012
Citation: Mustafa, F., Irfan Ullah, M., Kneeland, K.M., Coudron, T.A., Stanley, D.W., Hoback, W., Foster, J.E. 2012. The assessment of genetic variability among spined soldier bug (Pentatomidae: Hemiptera) populations [abstract]. Entomological Society of America Annual Meeting. p. 79.
Spined soldier bug Podisus maculiventris (Say) is a predatory hemipteran found in North America. It is an important biological control agent for agricultural and forest pests. This polyphagous predator mainly preys upon the eggs and larvae of the lepidopteran and coleopteran species. To assess the genetic variability among the populations from different states, the samples were collected from Florida, Mississippi, Michigan, Maryland and Missouri. The sample populations were evaluated by the polymerase chain reaction-amplified fragment length polymorphism (PCR-AFLP) technique. Four AFLP primer sets were used to generate molecular markers. The binary data were evaluated using Analysis of Molecular Variance (AMOVA) and Nei’s genetic diversity. The result showed that the most of the variations lie within populations.