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Title: Liquid Chromatographic Detection of Permethrin from Filter Paper Wipes of White-tailed Deer

Author
item Lohmeyer, Kimberly - Kim
item Pound, Joe
item Klavons, Jerome
item Davey, Ronald

Submitted to: Journal of Entomological Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/13/2013
Publication Date: 8/1/2013
Citation: Lohmeyer, K.H., Pound, J.M., Klavons, J.A., Davey, R.B. 2013. Liquid chromatographic detection of permethrin from filter paper wipes of white-tailed deer. Journal of Entomological Science. 48:258-260.

Interpretive Summary: A simple inexpensive method for determining the presence or absence of permethrin pesticide on the hair coat of white-tailed deer by liquid chromatography was developed. White-tailed deer in South Texas and the northeastern U.S. are routinely treated with permethrin to control lone star ticks, black legged ticks, cattle ticks, and southern cattle ticks. Filter paper wipes from captured deer were extracted in solvent for 24 h. The presence or absence of permethrin was then verified using liquid chromatography. This method was used to verify the proportion of white-tailed deer using tick treatment devices by determining the presence or absence of permethrin on the hair coat. Determining the proportion of the white-tailed deer population utilizing these treatment devices is critical for determining the efficiency of treatment of these types of devices, as well as for making decisions about the numbers of devices to put into the field.

Technical Abstract: A simple, small-scale method for the determination of the presence or absence of permethrin on the hair coat of white-tailed deer, Odocoileus virginianus (Zimmermann), by high performance liquid chromatography was developed. White-tailed deer in South Texas and the northeastern U.S. are routinely treated with permethrin to control lone star ticks, Amblyomma americanum (L.), black legged ticks, Ixodes scapularis Say, cattle ticks, Rhipicephalus (Boophilus) annulatus (Say), and southern cattle ticks, R. (B.) microplus (Canestrini). Sample filter paper wipes from net gun captured white-tailed deer were extracted in acetonitrile for 24 h. The presence or absence of permethrin was then verified using liquid chromatography with detection at 237 nm. This method was used to verify the proportion of white-tailed deer using ‘four-poster’ and ‘two-poster’ passive treatment devices by determining the presence or absence of permethrin on the hair coat. Determining the proportion of the white-tailed deer population utilizing these treatment devices is critical for determining the efficiency of treatment of these types of devices, as well as for making decisions about the numbers of devices to deploy in the field.