Technical Abstract: A good model plant to investigate plant – pathogen interactions would be easy to grow, have a short life cycle, be a natural host of many pathogens, and be easy to manipulate genetically. Hairy nightshade (Solanum sarrachoides) is a ubiquitous, fast growing weed that produces copious amounts of seed and grows commonly in many agricultural settings. It is a solanaceous plant related to potato, tomato, pepper and eggplant, and is a host of many of the principal fungal, bacterial and viral pathogens that affect these crops. The primary drawback is a lack of information on tissue culture methods for this plant. Effects on shoot regeneration were investigated based on the use of different gelling agents, cotyledon orientation, hypocotyl segments from different locations, and cytokinins. The type of gelling agent and tissue orientation had no effect on regeneration from cotyledons. However, effects on regeneration were observed based on the location of the hypocotyl segment, the type of cytokinin, and tissue type. Hypocotyl segments proximal to the cotyledons had a significant effect on regeneration. When benzylaminopurine or zeatin were used at various concentrations, the best shoot regeneration was found with 1 mg L-1 zeatin or 1mg L-1 BA. Shoots were easily rooted during prolonged incubation on the rooting media.