ARS | Publication request: Beneficial effects of neotyphodium tembladerae and neotyphodium pampeanum on a wild forage grass

Submitted to: Grass and Forage Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 12, 2011
Publication Date: March 28, 2012
Citation: Iannone, L.J., Pinget, A.D., Nagabhyru, P., Schardl, C.L., De Battista, J.P. 2012. Beneficial effects of Neotyphodium tembladerae and Neotyphodium pampeanum on a wild forage grass. Grass and Forage Science. 67:382-390.

Interpretive Summary: Asexual, vertically transmitted fungal endophytes of the genus Neotyphodium are considered to enhance growth, stress resistance and competitiveness of agronomic grasses, but have been suggested to have neutral or deleterious effects on wild grasses. We studied whether the associations between Bromus auleticus and their endophytes, Neotyphodium pampeanum and Neotyphodium tembladerae, are beneficial for this wild forage grass native to South America. In a greenhouse experiment, 3-month-old endophyte-infected plants (E+) showed enhanced growth relative to their endophyte-free counterparts (E-). In a one-year-long experiment in the field, E+ plants showed higher survival and regrowth rate, and produced more biomass and seeds than E- plants. Only with respect to the seed output did N. tembladerae seem to be detrimental, whereas N. pampeanum did not affect this parameter. No differences were observed with respect to the germination of seeds produced by E+ or E- plants. Loline alkaloids were detected in N. pampeanum-infected plants. Our results show that, similar to results with agronomic grasses, in wild grasses, the symbiosis with Neotyphodium species could be mutualistic.

Technical Abstract: Asexual, vertically transmitted fungal endophytes of the genus Neotyphodium are considered to enhance growth, stress resistance and competitiveness of agronomic grasses, but have been suggested to have neutral or deleterious effects on wild grasses. We studied whether the associations between Bromus auleticus and their endophytes, Neotyphodium pampeanum and Neotyphodium tembladerae, are beneficial for this wild forage grass native to South America. In a greenhouse experiment, 3-month-old endophyte-infected plants (E+) showed enhanced growth relative to their endophyte-free counterparts (E-). In a one-year-long experiment in the field, E+ plants showed higher survival and regrowth rate, and produced more biomass and seeds than E- plants. Only with respect to the seed output did N. tembladerae seem to be detrimental, whereas N. pampeanum did not affect this parameter. No differences were observed with respect to the germination of seeds produced by E+ or E- plants. Loline alkaloids were detected in N. pampeanum-infected plants. Our results show that, similar to results with agronomic grasses, in wild grasses, the symbiosis with Neotyphodium species could be mutualistic.