Technical Abstract: This is a review article summarizing genomic profiling strategies for determining the mechanism of action of antifungal synergies, and highlighting the potential applications of these technologies. Given the limitations of currently available antifungal agents and the development of drug resistance, there is an urgent need for discovering new antifungal drugs, in particular drugs that can be used in combination therapies. Identifying the mechanism behind drug synergies is not only an important step towards drug development, but it also has the potential to generate new pharmacological tools for further exploration of the targeted pathways and understanding how they interact with each other.