ARS | Publication request: Quantitative determination of triperpene saponins and alkenated-phenolics from Labisia pumila using LC-UV/ELSD method and confirmation by LC-ESI-TOF

Submitted to: Planta Medica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 1, 2011
Publication Date: May 17, 2011
Citation: Avula, B., Wang, Y., Ali, Z., Smillie, T.J., Khan, I.A. 2011. Quantitative determination of triperpene saponins and alkenated-phenolics from Labisia pumila using LC-UV/ELSD method and confirmation by LC-ESI-TOF. Planta Medica. 77:1742-1748.

Interpretive Summary: The newly developed HPLC-UV-ELSD method was found to be capable for the determination of saponins and alkenated phenolics from the aerial parts and roots of L. pumila. Ardisiacrispin A was found to be a major compound for all plant samples analyzed. The compounds were further confirmed by LC-ESI-TOF in plant samples. The developed method is simple, economic and especially suitable for quality control analysis of products.

Technical Abstract: This study describes the first analytical method for the determination of saponins and alkenated-phenolics from the leaves, leaves/stems and roots of Labisia pumila using a HPLC-UV-ELSD method. The separation was achieved using a reversed phase column, PDA and ELS detection, and a water/acetonitrile gradient as the mobile phase. Analysis of various samples showed considerable variation of 0.11-2.46% for the major triterpenoid compound, ardisiacrispin A. LC-mass spectrometry method coupled with ESI is described for the identification of compounds in plant samples.