COUNTERMEASURES TO PREVENT, MITIGATE, AND CONTROL RIFT VALLEY FEVER (RVF)
Location: Arthropod-Borne Animal Diseases Research
Title: Design of a shear-thinning recoverable peptide hydrogel from native sequences and application for influenza H1N1 vaccine adjuvant
| Huang, Hongzhou - |
| Shi, Jishu - |
| Laskin, Julia - |
| Liu, Ziyan - |
McVey, D Scott
| Sun, Xiuzhi - |
Submitted to: Soft Matters
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 25, 2011
Publication Date: July 21, 2011
Citation: Huang, H., Shi, J., Laskin, J., Liu, Z., Mcvey, D.S., Sun, X.S. 2011. Design of a shear-thinning recoverable peptide hydrogel from native sequences and application for influenza H1N1 vaccine adjuvant. Soft Matters.
Interpretive Summary: A self-assembling peptide was designed and synthesized by rationally combining two native sequences from an elastic segment of spider silk and a trans-membrane segment of human muscle L-type calcium channel. The peptide forms two distinct hydrogels in Ca2+ solution and acidic pH conditions. The shear-thinning, rapid-strength-recovering Ca2+ hydrogel has great potential for drug delivery and tissue engineering applications, for example, as an H1N1 influenza vaccine adjuvant.
Peptide hydrogels are considered injectable materials for drug delivery and tissue engineering applications. Most published hydrogel-forming sequences contain either alternating-charged and noncharged residues or amphiphilic blocks. Here, we report a self-assembling peptide, h9e (FLIVIGSIIGPGGDGPGGD), designed by rationally combining two native sequences from an elastic segment of spider silk and a trans-membrane segment of human muscle L-type calcium channel. The turning segment GSII of h9e promoted hydrogel formation in both Ca2+ solution and acidic pH conditions at water content greater than 99.5%. Although h9e Ca2+ hydrogel and h9e acidic hydrogel have the same sequence, they have distinct physical properties. The shear-thinning, rapid-strength-recovering h9e Ca2+ hydrogel was used as an H1N1 influenza vaccine adjuvant. The h9e adjuvant was biologically safe and improved immune response by _70% compared with an oil-based commercial adjuvant.