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United States Department of Agriculture

Agricultural Research Service

Research Project: COUNTERMEASURES TO PREVENT, MITIGATE, AND CONTROL RIFT VALLEY FEVER (RVF)

Location: Arthropod-Borne Animal Diseases Research

Title: Design of a shear-thinning recoverable peptide hydrogel from native sequences and application for influenza H1N1 vaccine adjuvant

Authors
item Huang, Hongzhou -
item Shi, Jishu -
item Laskin, Julia -
item Liu, Ziyan -
item McVey, D Scott
item Sun, Xiuzhi -

Submitted to: Soft Matters
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 25, 2011
Publication Date: July 21, 2011
Repository URL: http://pubs.rsc.org/en/content/articlepdf/2011/sm/c1sm05157a
Citation: Huang, H., Shi, J., Laskin, J., Liu, Z., Mcvey, D.S., Sun, X.S. 2011. Design of a shear-thinning recoverable peptide hydrogel from native sequences and application for influenza H1N1 vaccine adjuvant. Soft Matters.

Interpretive Summary: A self-assembling peptide was designed and synthesized by rationally combining two native sequences from an elastic segment of spider silk and a trans-membrane segment of human muscle L-type calcium channel. The peptide forms two distinct hydrogels in Ca2+ solution and acidic pH conditions. The shear-thinning, rapid-strength-recovering Ca2+ hydrogel has great potential for drug delivery and tissue engineering applications, for example, as an H1N1 influenza vaccine adjuvant.

Technical Abstract: Peptide hydrogels are considered injectable materials for drug delivery and tissue engineering applications. Most published hydrogel-forming sequences contain either alternating-charged and noncharged residues or amphiphilic blocks. Here, we report a self-assembling peptide, h9e (FLIVIGSIIGPGGDGPGGD), designed by rationally combining two native sequences from an elastic segment of spider silk and a trans-membrane segment of human muscle L-type calcium channel. The turning segment GSII of h9e promoted hydrogel formation in both Ca2+ solution and acidic pH conditions at water content greater than 99.5%. Although h9e Ca2+ hydrogel and h9e acidic hydrogel have the same sequence, they have distinct physical properties. The shear-thinning, rapid-strength-recovering h9e Ca2+ hydrogel was used as an H1N1 influenza vaccine adjuvant. The h9e adjuvant was biologically safe and improved immune response by _70% compared with an oil-based commercial adjuvant.

Last Modified: 7/30/2014
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