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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOP METHODS TO ASSESS AND IMPROVE POULTRY AND EGG QUALITY

Location: Quality and Safety Assessment Research Unit

Title: Analysis of phenolic compounds from corn, oat, and wheat bran extracts by LC-MS-PDA

Authors
item Holser, Ronald
item Hawkins, Samantha
item Gamble, Gary

Submitted to: Montreux Symposium on Liquid Chromatography/Mass Spectrometry
Publication Type: Abstract Only
Publication Acceptance Date: July 4, 2012
Publication Date: November 7, 2012
Citation: Holser, R.A., Hawkins, S.A., Gamble, G.R. 2012. Analysis of phenolic compounds from corn, oat, and wheat bran extracts by LC-MS-PDA [abstract]. Montreux Symposium on Liquid Chromatography/Mass Spectrometry. 2012 CDROM.

Interpretive Summary: Phenolic compounds are among the most common secondary metabolites produced by plants and can exhibit a range of bioactive properties including antimicrobial, antioxidant, and antihypertensive. These natural products have applications in nutraceutical, pharmaceutical and functional food or animal feed formulations. The combination of mass spectrometry with ultraviolet (UV) spectroscopy was used to quickly characterize the phenolic compounds found in extracts from corn, oat, and wheat brans. Extracts were analyzed with an Accela UHPLC-MSQ-PDA system (ThermoFisher Scientific, Waltham, Massachusetts, USA). Separations were performed using a C18 column. The MS detector scanned from 50 – 500 m/z and was operated with in negative mode with electrospray interface. The diode array detector collected UV spectra between 200 and 400 nm. The MS and UV results were correlated to quickly profile the phenolic compounds in an extract and used to guide the selection of extraction conditions.

Technical Abstract: Phenolic compounds are among the most common secondary metabolites produced by plants and can exhibit a range of bioactive properties including antimicrobial, antioxidant, and antihypertensive. These natural products have applications in nutraceutical, pharmaceutical and functional food or animal feed formulations. The combination of mass spectrometry with ultraviolet (UV) spectroscopy was used to quickly characterize the phenolic compounds found in extracts from corn, oat, and wheat brans. Extracts were analyzed with an Accela UHPLC-MSQ-PDA system (ThermoFisher Scientific, Waltham, Massachusetts, USA). Separations were performed using a C18 column. The MS detector scanned from 50 – 500 m/z and was operated with in negative mode with electrospray interface. The diode array detector collected UV spectra between 200 and 400 nm. The MS and UV results were correlated to quickly profile the phenolic compounds in an extract and used to guide the selection of extraction conditions.

Last Modified: 4/17/2014